A rapid and simple method for DNA engineering using cycled ligation assembly.

A rapid and simple method for DNA engineering using cycled ligation assembly.

DNA assembly techniques have developed rapidly, enabling efficient construction of complex constructs that would be prohibitively difficult using traditional restriction-digest based methods. Most of the recent methods for assembling multiple DNA fragments in vitro suffer from high costs, complex se...

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Bibliographic Details
Main Authors: Theodore L Roth, Ljiljana Milenkovic, Matthew P Scott
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4167330?pdf=render

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