Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification

Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification

The experience with a diagnostic technology based on rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) analyses, and direct or deep sequencing (Circomics) over the past 15 years is surveyed for the plant infecting geminiviruses, nanoviruses and associated satellite...

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Bibliographic Details
Main Author: Holger Jeske
Format: Article
Language:English
Published: MDPI AG 2018-08-01
Series:Viruses
Subjects:
geminivirus
nanovirus
satellite
RCA
RFLP
Circomics
Online Access:http://www.mdpi.com/1999-4915/10/9/469
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spelling doaj-88e6f75d810042e09f681ef6f66a3b1e2020-11-24T21:23:20ZengMDPI AGViruses1999-49152018-08-0110946910.3390/v10090469v10090469Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle AmplificationHolger Jeske0Department of Molecular Biology and Plant Virology, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart, GermanyThe experience with a diagnostic technology based on rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) analyses, and direct or deep sequencing (Circomics) over the past 15 years is surveyed for the plant infecting geminiviruses, nanoviruses and associated satellite DNAs, which have had increasing impact on agricultural and horticultural losses due to global transportation and recombination-aided diversification. Current state methods for quarantine measures are described to identify individual DNA components with great accuracy and to recognize the crucial role of the molecular viral population structure as an important factor for sustainable plant protection.http://www.mdpi.com/1999-4915/10/9/469geminivirusnanovirussatelliteRCARFLPCircomics
collection DOAJ
language English
format Article
sources DOAJ
author Holger Jeske
spellingShingle Holger Jeske
Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification
Viruses
geminivirus
nanovirus
satellite
RCA
RFLP
Circomics
author_facet Holger Jeske
author_sort Holger Jeske
title Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification
title_short Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification
title_full Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification
title_fullStr Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification
title_full_unstemmed Barcoding of Plant Viruses with Circular Single-Stranded DNA Based on Rolling Circle Amplification
title_sort barcoding of plant viruses with circular single-stranded dna based on rolling circle amplification
publisher MDPI AG
series Viruses
issn 1999-4915
publishDate 2018-08-01
description The experience with a diagnostic technology based on rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) analyses, and direct or deep sequencing (Circomics) over the past 15 years is surveyed for the plant infecting geminiviruses, nanoviruses and associated satellite DNAs, which have had increasing impact on agricultural and horticultural losses due to global transportation and recombination-aided diversification. Current state methods for quarantine measures are described to identify individual DNA components with great accuracy and to recognize the crucial role of the molecular viral population structure as an important factor for sustainable plant protection.
topic geminivirus
nanovirus
satellite
RCA
RFLP
Circomics
url http://www.mdpi.com/1999-4915/10/9/469
work_keys_str_mv AT holgerjeske barcodingofplantviruseswithcircularsinglestrandeddnabasedonrollingcircleamplification
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