Detection of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum by Multiplex PCR in Semen Sample of Infertile Men

• Main Authors: M Golshani, G Eslami, Sh Mohhammadzadeh Ghobadloo, F Fallah, H Goudarzi, AA Soleimani Rahbar, F Fayaz
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2007-08-01
• Series: Iranian Journal of Public Health
• Subjects: Male
• Online Access: http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/3534.pdf&manuscript_id=3534
• ISSN: 2251-6085

Background: The aim of this study was to detect Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyti¬cum from semen samples of infertile men by Multiplex PCR and investigation of influence of bacteriospermia on semen parame¬ters. Methods: Semen samples of 200 infertile men were evaluated by Multiplex PCR. In addition, analysis of semen parameters was performed according to the WHO guidelines. Results: All the patients were without clinical symptoms of urogenital tract infection. Thirty three percent of cases showed at least one bacterium. We found a noticeable relation between the presence of bacteriospermia and the rate of non motile and morphologically abnormal sperms (P 0.05). Conclusion: Asymptomatic existence of Chlamydia and Mycoplasmas in urogenital tracts might play an important role in sperm impairment due to infertility. Bacteriospermia can influence sperm's motility, morphology and concentration.