A Single-Batch Fermentation System to Simulate Human Colonic Microbiota for High-Throughput Evaluation of Prebiotics.

We devised a single-batch fermentation system to simulate human colonic microbiota from fecal samples, enabling the complex mixture of microorganisms to achieve densities of up to 1011 cells/mL in 24 h. 16S rRNA gene sequence analysis of bacteria grown in the system revealed that representatives of...

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Bibliographic Details
Main Authors: Risa Takagi, Kengo Sasaki, Daisuke Sasaki, Itsuko Fukuda, Kosei Tanaka, Ken-Ichi Yoshida, Akihiko Kondo, Ro Osawa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4970706?pdf=render