Novel Cell-Ess ® supplement used as a feed or as an initial boost to CHO serum free media results in a significant increase in protein yield and production

• Main Author: Adam Elhofy
• Format: Article
• Language: English
• Published: Elsevier 2016-01-01
• Series: Computational and Structural Biotechnology Journal
• Subjects: Bioprocessing; Titer; Lipid; Cell-Ess; CHO; Monoclonal antibody; Serum free media; Animal component free; Viable cell density; Bioreactor; Single use bioreactor
• Online Access: http://www.sciencedirect.com/science/article/pii/S200103701630023X

Many metrics, including metabolic profiles, have been used to analyze cell health and optimize productivity. In this study, we investigated the ability of a lipid supplement to increase protein yield. At a concentration of 1% (v/v) the lipid supplement caused a significant increase in protein titer (1118 ± 65.4 ng 105 cells−1 days−1) when compared to cultures grown in the absence of supplementation (819.3 ± 38.1 ng 105 cells−1 days−1; p < 0.05). This equated to a 37% increase in productivity. Furthermore, metabolic profiles of ammonia, glutamate, lactate, and glucose were not significantly altered by the polar lipid supplement. In a separate set of experiments, using the supplement as a feed resulted in 2 notable effects. The first was a 25% increase in protein titer. The second was an extension of peak protein production from 1 day to 2 days. These results suggest that lipid supplementation is a promising avenue for enhancing protein production. In addition, our results also suggest that an increase in protein production may not necessarily require a change in the metabolic state of the cells.