Profiling soil microbial communities with next-generation sequencing: the influence of DNA kit selection and technician technical expertise
Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles in the ecology of various environments. Different DNA isolation protocols can lead to data bias and can affect results of next-generation sequencing. To evaluate the impac...
Main Authors: | Taha Soliman, Sung-Yin Yang, Tomoko Yamazaki, Holger Jenke-Kodama |
---|---|
Format: | Article |
Language: | English |
Published: |
PeerJ Inc.
2017-12-01
|
Series: | PeerJ |
Subjects: | |
Online Access: | https://peerj.com/articles/4178.pdf |
Similar Items
-
Microbial composition of spiny ants (Hymenoptera: Formicidae: Polyrhachis) across their geographic range
by: Manuela Oliveira Ramalho, et al.
Published: (2017-04-01) -
Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions
by: Eun Young Lee, et al.
Published: (2020-04-01) -
Imobilização de enzimas a partir de "kit" comercial: determinação de parâmetros metabólicos em sangue animal empregando multicomutação em fluxo Enzyme immobilization using a commercial kit: determination of metabolic parameters in animal blood employing a multicommutation flow system
by: Cherrine Kelce Pires, et al.
Published: (2005-06-01) -
Comparison of Commercial ELISA Kits to Confirm the Absence of Transmission in Malaria Elimination Settings
by: Lotus L. van den Hoogen, et al.
Published: (2020-09-01) -
Evaluation of three <em>Brettanomyces</em> qPCR commercial kits: results from an interlaboratory study
by: Cédric Longin, et al.
Published: (2016-12-01)