In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability

In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability

Cell-based therapy using adult mesenchymal stem cells (MSCs) has already been the subject of clinical trials, but for further development and optimization the distribution and integration of the engrafted cells into host tissues have to be monitored. Today, for this purpose magnetic resonance imagin...

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Main Authors: K. Nohroudi, S. Arnhold, T. Berhorn, K. Addicks, M. Hoehn, U. Himmelreich
Format: Article
Language:English
Published: SAGE Publishing 2010-04-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/096368909X484699
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spelling doaj-88714729f9384196a1b2a188343c1ef72020-11-25T04:01:10ZengSAGE PublishingCell Transplantation0963-68971555-38922010-04-011910.3727/096368909X484699In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell ViabilityK. Nohroudi0S. Arnhold1T. Berhorn2K. Addicks3M. Hoehn4U. Himmelreich5 Department of Anatomy I, University of Cologne, Cologne, Germany Department of Veterinary Anatomy, University of Giessen, Giessen, Germany Department of Anatomy I, University of Cologne, Cologne, Germany Department of Anatomy I, University of Cologne, Cologne, Germany Max Planck Institute for Neurological Research, Cologne, Germany Max Planck Institute for Neurological Research, Cologne, GermanyCell-based therapy using adult mesenchymal stem cells (MSCs) has already been the subject of clinical trials, but for further development and optimization the distribution and integration of the engrafted cells into host tissues have to be monitored. Today, for this purpose magnetic resonance imaging (MRI) is the most suitable technique, and micron-sized iron oxide particles (MPIOs) used for labeling are favorable due to their low detection limit. However, constitutional data concerning labeling efficiency, cell viability, and function are lacking. We demonstrate that cell viability and migratory potential of bone marrow mesenchymal stromal cells (BMSCs) are negatively correlated with incorporated MPIOs, presumably due to interference with the actin cytoskeleton. Nevertheless, labeling of BMSCs with low amounts of MPIOs results in maintained cellular function and sufficient contrast for in vivo observation of single cells by MRI in a rat glioma model. Conclusively, though careful titration is indicated, MPIOs are a promising tool for in vivo cell tracking and evaluation of cell-based therapies.https://doi.org/10.3727/096368909X484699
collection DOAJ
language English
format Article
sources DOAJ
author K. Nohroudi
S. Arnhold
T. Berhorn
K. Addicks
M. Hoehn
U. Himmelreich
spellingShingle K. Nohroudi
S. Arnhold
T. Berhorn
K. Addicks
M. Hoehn
U. Himmelreich
In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability
Cell Transplantation
author_facet K. Nohroudi
S. Arnhold
T. Berhorn
K. Addicks
M. Hoehn
U. Himmelreich
author_sort K. Nohroudi
title In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability
title_short In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability
title_full In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability
title_fullStr In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability
title_full_unstemmed In Vivo MRI Stem Cell Tracking Requires Balancing of Detection Limit and Cell Viability
title_sort in vivo mri stem cell tracking requires balancing of detection limit and cell viability
publisher SAGE Publishing
series Cell Transplantation
issn 0963-6897
1555-3892
publishDate 2010-04-01
description Cell-based therapy using adult mesenchymal stem cells (MSCs) has already been the subject of clinical trials, but for further development and optimization the distribution and integration of the engrafted cells into host tissues have to be monitored. Today, for this purpose magnetic resonance imaging (MRI) is the most suitable technique, and micron-sized iron oxide particles (MPIOs) used for labeling are favorable due to their low detection limit. However, constitutional data concerning labeling efficiency, cell viability, and function are lacking. We demonstrate that cell viability and migratory potential of bone marrow mesenchymal stromal cells (BMSCs) are negatively correlated with incorporated MPIOs, presumably due to interference with the actin cytoskeleton. Nevertheless, labeling of BMSCs with low amounts of MPIOs results in maintained cellular function and sufficient contrast for in vivo observation of single cells by MRI in a rat glioma model. Conclusively, though careful titration is indicated, MPIOs are a promising tool for in vivo cell tracking and evaluation of cell-based therapies.
url https://doi.org/10.3727/096368909X484699
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AT kaddicks invivomristemcelltrackingrequiresbalancingofdetectionlimitandcellviability
AT mhoehn invivomristemcelltrackingrequiresbalancingofdetectionlimitandcellviability
AT uhimmelreich invivomristemcelltrackingrequiresbalancingofdetectionlimitandcellviability
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