The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum

The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum

The use of polyazolidinammonium modified with iodine hydrate ions (PAAG) as an adjuvant made it possible to obtain rabbit hyperimmune blood serums for dimethyl-sulfoxide antigen (DA) of a pseudotuberculosis microbe with generic specificity. Antibody titers in ELISA with Y. pseudotuberculosis and Y....

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Main Authors: Manieson Victor, Ivaschenko Sergey
Format: Article
Language:English
Published: EDP Sciences 2020-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/35/e3sconf_interagromash2020_03011.pdf
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spelling doaj-885cda4103ec442e98e7696ce22223742021-04-02T14:31:33ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011750301110.1051/e3sconf/202017503011e3sconf_interagromash2020_03011The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serumManieson Victor0Ivaschenko Sergey1Saratov State Agrarian University named after N.I. VavilovaSaratov State Agrarian University named after N.I. VavilovaThe use of polyazolidinammonium modified with iodine hydrate ions (PAAG) as an adjuvant made it possible to obtain rabbit hyperimmune blood serums for dimethyl-sulfoxide antigen (DA) of a pseudotuberculosis microbe with generic specificity. Antibody titers in ELISA with Y. pseudotuberculosis and Y. enterocolitica cells amounted to 1:25600-1:12800, and with cells of other genera of intestinal microflora 1:100-1:400. The optimal immunizing dose for obtaining hyperimmune yersiniosis serum was a dose of 2 mg DA of Y. pseudotuberculosis per rabbit. Such a dose made it possible to obtain hyperimmune sera with a high titer of specific antibodies with a small consumption of antigen. The optimal concentration of PAAG solution for hyperimmunization of Y. pseudotuberculosis DA rabbits was 1%.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/35/e3sconf_interagromash2020_03011.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Manieson Victor
Ivaschenko Sergey
spellingShingle Manieson Victor
Ivaschenko Sergey
The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
E3S Web of Conferences
author_facet Manieson Victor
Ivaschenko Sergey
author_sort Manieson Victor
title The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
title_short The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
title_full The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
title_fullStr The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
title_full_unstemmed The use of polyazolidineammonium and dimethyl-sulfoxide antigen Yersinia pseudotuberculosis to obtain hyperimmune serum
title_sort use of polyazolidineammonium and dimethyl-sulfoxide antigen yersinia pseudotuberculosis to obtain hyperimmune serum
publisher EDP Sciences
series E3S Web of Conferences
issn 2267-1242
publishDate 2020-01-01
description The use of polyazolidinammonium modified with iodine hydrate ions (PAAG) as an adjuvant made it possible to obtain rabbit hyperimmune blood serums for dimethyl-sulfoxide antigen (DA) of a pseudotuberculosis microbe with generic specificity. Antibody titers in ELISA with Y. pseudotuberculosis and Y. enterocolitica cells amounted to 1:25600-1:12800, and with cells of other genera of intestinal microflora 1:100-1:400. The optimal immunizing dose for obtaining hyperimmune yersiniosis serum was a dose of 2 mg DA of Y. pseudotuberculosis per rabbit. Such a dose made it possible to obtain hyperimmune sera with a high titer of specific antibodies with a small consumption of antigen. The optimal concentration of PAAG solution for hyperimmunization of Y. pseudotuberculosis DA rabbits was 1%.
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/35/e3sconf_interagromash2020_03011.pdf
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AT ivaschenkosergey theuseofpolyazolidineammoniumanddimethylsulfoxideantigenyersiniapseudotuberculosistoobtainhyperimmuneserum
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