Optimization of Cultural Conditions for Production of Antibacterial Metabolites from <i>Streptomyces coelicoflavus</i> BC 01

• Main Authors: Kothagorla Venkata RAGHAVA RAO, Dadi BHASKARA RAO, Botcha SATYANARAYANA, Tamanam RAGHAVA RAO
• Format: Article
• Language: English
• Published: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 2015-06-01
• Series: Notulae Scientia Biologicae
• Online Access: http://notulaebiologicae.ro/index.php/nsb/article/view/9481

<p class="Continutabstract">The aim of the present study was to optimize various cultural conditions for the production of antibacterial metabolites by <em>Streptomyces coelicoflavus</em> BC 01 isolated from mangrove soil, Visakhapatnam, Andhra Pradesh, India. The effect of various factors such as carbon and nitrogen sources, different concentrations of NaCl and K₂HPO₄, different temperature, pH, incubation time and agitation on antibacterial metabolites production were studied. The production of antibacterial metabolites by the isolate <em>Streptomyces coelicoflavus</em> BC 01 was greatly influenced by the cultural conditions. Glucose (1.2%) and soya bean meal (1%) seemed to be the best carbon and nitrogen source respectively, followed by NaCl (1%) and K₂HPO₄(0.25%). Maximum production of antibacterial metabolites was observed at a temperature of 30 °C, with pH 7.2, at 160 rpm for 96 hrs. These optimized parameters can be further useful to design a fermentation medium to achieve maximum yield of antibacterial metabolites from <em>Streptomyces coelicoflavus</em> BC 01.</p>