Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population

Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population

A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription fact...

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Main Authors: Wenxin Zhang, Qiuting Dai, Yishi Hua, Weihao Di, Jinghui Guo, Jian Zhao, Yulei Deng, Ying Wang
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121000180
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spelling doaj-882c1d5853c74893ad1c1a27656c30b32021-03-13T04:22:14ZengElsevierStem Cell Research1873-50612021-03-0151102172Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han populationWenxin Zhang0Qiuting Dai1Yishi Hua2Weihao Di3Jinghui Guo4Jian Zhao5Yulei Deng6Ying Wang7Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaNeurology Department, Shanghai Ruijin Hospital, Jiaotong University School of Medicine, Shanghai, China; Corresponding authors.Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The iPSC line displayed a normal karyotype. This model provides insight into further pathological studies to research identify early biomarkers, study disease pedigrees, and also for drug testing purposes.http://www.sciencedirect.com/science/article/pii/S1873506121000180
collection DOAJ
language English
format Article
sources DOAJ
author Wenxin Zhang
Qiuting Dai
Yishi Hua
Weihao Di
Jinghui Guo
Jian Zhao
Yulei Deng
Ying Wang
spellingShingle Wenxin Zhang
Qiuting Dai
Yishi Hua
Weihao Di
Jinghui Guo
Jian Zhao
Yulei Deng
Ying Wang
Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
Stem Cell Research
author_facet Wenxin Zhang
Qiuting Dai
Yishi Hua
Weihao Di
Jinghui Guo
Jian Zhao
Yulei Deng
Ying Wang
author_sort Wenxin Zhang
title Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
title_short Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
title_full Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
title_fullStr Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
title_full_unstemmed Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population
title_sort generation of three ipsc cell lines (siaisi006-a, siaisi007-a and siaisi008-a) from a 66-year-old alzheimer’s disease (ad) patient and her two unaffected children from chinese han population
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-03-01
description A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The iPSC line displayed a normal karyotype. This model provides insight into further pathological studies to research identify early biomarkers, study disease pedigrees, and also for drug testing purposes.
url http://www.sciencedirect.com/science/article/pii/S1873506121000180
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