Effect of Oral Consumption of Magnesium on Glucose Concentration and Formalin Test in Diabetic Rats

• Main Authors: N Gheibi, H Rasolpor, F Rajaei, H Jahani Hashemi, MH Esmaeili, E Abbasi
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2008-07-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: Diabetes Mellitus; Magnesium; Formalin test; pain; Rat
• Online Access: http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-39-26&slc_lang=en&sid=1
• ISSN: 1728-6506; 1728-6514

Introduction & Objective: Magnesium is an essential metal in carbohydrate metabolism that causes activation and release of insulin. Increasing of blood sugar in diabetic patients resulted in decreasing of magnesium in the serum and its low concentration in urine. The aim of this study was to evaluate the effect of oral administration of magnesium on glucose concentration and formalin test in diabetic rats. Materials & Methods: This experimental study was done from April 2007 till July 2008 in medical school of Qazvin University of Medical Sciences. In this study 80 NMRI rats (Razi Co. Iran) categorized in ten groups control, diabetic without treatment of magnesium, diabetic with treatment of magnesium in one (1), two (2), three (3) and four (4) week(s). The other 4 normal groups were treated with magnesium in same timescales. In the diabetic groups, diabetes was induced with i.p. injection of 60mg/kg of streptozotocin. Besides of weight measurements, glucose concentration of animals was measured with enzymaticcolorimetric method. Pain scores were measured in formalin test. Statistical analysis was carried out by ANOVA and Tukey test. Results: The results showed that the difference in animal weight and glucose concentrations between control and diabetic groups was significant (P<0.0001). Glucose concentrations of magnesium treated diabetic groups were significantly different between 1 and 2 (P<0.005) 1 and 3 or 4 groups (P<0.0001) 2 and 3 (P<0.001) and 2 and 4 (P<0.01). Statistical differences between control and magnesium treated diabetic groups were significant between control and 1 (P<0.0001) control and 2 (P<0.05). Considering the weight of rats, there was no significant difference between diabetic in one side and 1 and 2 groups in other side while differences between diabetic and the other 3 and 4 groups were significant (P<0.001 and P<0.0001, respectively). In this study results from flinching and licking responses have been evoked by formalin in biphasic model of formalin test. Conclusion: Magnesium consuming in diabetic rats resulted in time dependent increasing of animals weight and decreasing of glucose concentration. Results from formalin test show hyperalgesic effects in diabetic and diabetic treated with magnesium groups in comparison with control groups.