The kin17 Protein in Murine Melanoma Cells

The kin17 Protein in Murine Melanoma Cells

kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line...

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Main Authors: Anelise C. Ramos, Vanessa P. Gaspar, Sabrina M. G. Kelmer, Tarciso A. Sellani, Ana G. U. Batista, Quirino A. De Lima Neto, Elaine G. Rodrigues, Maria A. Fernandez
Format: Article
Language:English
Published: MDPI AG 2015-11-01
Series:International Journal of Molecular Sciences
Subjects:
kin17 protein
melanoma cells
DNA metabolism
Online Access:http://www.mdpi.com/1422-0067/16/11/26072
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spelling doaj-881453e668644babb5bec11c35a5b0552020-11-25T02:43:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-11-011611279122792010.3390/ijms161126072ijms161126072The kin17 Protein in Murine Melanoma CellsAnelise C. Ramos0Vanessa P. Gaspar1Sabrina M. G. Kelmer2Tarciso A. Sellani3Ana G. U. Batista4Quirino A. De Lima Neto5Elaine G. Rodrigues6Maria A. Fernandez7Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, BrazilLaboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, BrazilLaboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, BrazilLaboratório de Imunobiologia do Câncer, Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04023-062 São Paulo, São Paulo, BrazilDepartamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900 Maringá, Paraná, Brazilkin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.http://www.mdpi.com/1422-0067/16/11/26072kin17 proteinmelanoma cellsDNA metabolism
collection DOAJ
language English
format Article
sources DOAJ
author Anelise C. Ramos
Vanessa P. Gaspar
Sabrina M. G. Kelmer
Tarciso A. Sellani
Ana G. U. Batista
Quirino A. De Lima Neto
Elaine G. Rodrigues
Maria A. Fernandez
spellingShingle Anelise C. Ramos
Vanessa P. Gaspar
Sabrina M. G. Kelmer
Tarciso A. Sellani
Ana G. U. Batista
Quirino A. De Lima Neto
Elaine G. Rodrigues
Maria A. Fernandez
The kin17 Protein in Murine Melanoma Cells
International Journal of Molecular Sciences
kin17 protein
melanoma cells
DNA metabolism
author_facet Anelise C. Ramos
Vanessa P. Gaspar
Sabrina M. G. Kelmer
Tarciso A. Sellani
Ana G. U. Batista
Quirino A. De Lima Neto
Elaine G. Rodrigues
Maria A. Fernandez
author_sort Anelise C. Ramos
title The kin17 Protein in Murine Melanoma Cells
title_short The kin17 Protein in Murine Melanoma Cells
title_full The kin17 Protein in Murine Melanoma Cells
title_fullStr The kin17 Protein in Murine Melanoma Cells
title_full_unstemmed The kin17 Protein in Murine Melanoma Cells
title_sort kin17 protein in murine melanoma cells
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2015-11-01
description kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10CR. Nuclear and chromatin-associated protein fractions were analyzed, and kin17 was detected in all fractions, with an elevated concentration observed in the chromatin-associated fraction of the clone with low metastatic potential, suggesting that the kin17 expression level could be a marker of melanoma.
topic kin17 protein
melanoma cells
DNA metabolism
url http://www.mdpi.com/1422-0067/16/11/26072
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