Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches
Abstract Spectroscopic methods for quantitative detection of molecules of the heme synthesis and heme metabolism are presented. This can be used to diagnose disease patterns such as iron deficiency, porphyria or free hemoglobin. This article combines an overview of clinical conditions associated wit...
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Wiley-VCH
2021-05-01
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| Series: | Translational Biophotonics |
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| Online Access: | https://doi.org/10.1002/tbio.202000026 |
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doaj-880a540c572a439cb44f7536a98280b72021-06-25T07:33:01ZengWiley-VCHTranslational Biophotonics2627-18502021-05-0132n/an/a10.1002/tbio.202000026Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approachesChristian Heckl0Maximilian Eisel1Alexander Lang2Christian Homann3Michael Paal4Michael Vogeser5Adrian Rühm6Ronald Sroka7Laser‐Forschungslabor, LIFE Center, Department of Urology University Hospital, LMU Munich Munich GermanyLaser‐Forschungslabor, LIFE Center, Department of Urology University Hospital, LMU Munich Munich GermanyLaser‐Forschungslabor, LIFE Center, Department of Urology University Hospital, LMU Munich Munich GermanyLaser‐Forschungslabor, LIFE Center, Department of Urology University Hospital, LMU Munich Munich GermanyInstitute of Laboratory Medicine University Hospital, LMU Munich Munich GermanyInstitute of Laboratory Medicine University Hospital, LMU Munich Munich GermanyLaser‐Forschungslabor, LIFE Center, Department of Urology University Hospital, LMU Munich Munich GermanyLaser‐Forschungslabor, LIFE Center, Department of Urology University Hospital, LMU Munich Munich GermanyAbstract Spectroscopic methods for quantitative detection of molecules of the heme synthesis and heme metabolism are presented. This can be used to diagnose disease patterns such as iron deficiency, porphyria or free hemoglobin. This article combines an overview of clinical conditions associated with disorders of heme biosynthesis and presents selected laboratory findings in the development of portable diagnostic devices. An iron deficiency measurement device has already been validated as a portable device in clinical trials, two other methods have been tested according to EMA guidelines and are available for use in clinical validation studies. The presented rapid, low‐cost and low‐effort methods can support diagnosis in everyday clinical practice and initiate more targeted therapies, even for rare disease conditions.https://doi.org/10.1002/tbio.202000026fluorescence diagnosticsfree hemoglobiniron deficiencypoint of careporphyriasecond derivative evaluation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Christian Heckl Maximilian Eisel Alexander Lang Christian Homann Michael Paal Michael Vogeser Adrian Rühm Ronald Sroka |
| spellingShingle |
Christian Heckl Maximilian Eisel Alexander Lang Christian Homann Michael Paal Michael Vogeser Adrian Rühm Ronald Sroka Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches Translational Biophotonics fluorescence diagnostics free hemoglobin iron deficiency point of care porphyria second derivative evaluation |
| author_facet |
Christian Heckl Maximilian Eisel Alexander Lang Christian Homann Michael Paal Michael Vogeser Adrian Rühm Ronald Sroka |
| author_sort |
Christian Heckl |
| title |
Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches |
| title_short |
Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches |
| title_full |
Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches |
| title_fullStr |
Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches |
| title_full_unstemmed |
Spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: A review of laboratory work and point‐of‐care approaches |
| title_sort |
spectroscopic methods to quantify molecules of the heme‐biosynthesis pathway: a review of laboratory work and point‐of‐care approaches |
| publisher |
Wiley-VCH |
| series |
Translational Biophotonics |
| issn |
2627-1850 |
| publishDate |
2021-05-01 |
| description |
Abstract Spectroscopic methods for quantitative detection of molecules of the heme synthesis and heme metabolism are presented. This can be used to diagnose disease patterns such as iron deficiency, porphyria or free hemoglobin. This article combines an overview of clinical conditions associated with disorders of heme biosynthesis and presents selected laboratory findings in the development of portable diagnostic devices. An iron deficiency measurement device has already been validated as a portable device in clinical trials, two other methods have been tested according to EMA guidelines and are available for use in clinical validation studies. The presented rapid, low‐cost and low‐effort methods can support diagnosis in everyday clinical practice and initiate more targeted therapies, even for rare disease conditions. |
| topic |
fluorescence diagnostics free hemoglobin iron deficiency point of care porphyria second derivative evaluation |
| url |
https://doi.org/10.1002/tbio.202000026 |
| work_keys_str_mv |
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