Using Vital Dyes to Trace Uptake of dsRNA by Green Peach Aphid Allows Effective Assessment of Target Gene Knockdown

Using Vital Dyes to Trace Uptake of dsRNA by Green Peach Aphid Allows Effective Assessment of Target Gene Knockdown

RNA interference (RNAi) is an effective tool to study gene function. For in vitro studies of RNAi in insects, microinjection of double-stranded (ds)RNA may cause stress. Non-persuasive oral delivery of dsRNA to trigger RNAi is a better mode of delivery for delicate insects such as aphids because it...

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Bibliographic Details
Main Authors: Vineeta Bilgi, John Fosu-Nyarko, Michael G. K. Jones
Format: Article
Language:English
Published: MDPI AG 2017-01-01
Series:International Journal of Molecular Sciences
Subjects:
RNA interference (RNAi)
double stranded RNA (dsRNA)
green peach aphid
Myzus persicae
vacuolar (H+)-ATPase (vha-8)
vital dyes
neutral red
acridine orange
Online Access:http://www.mdpi.com/1422-0067/18/1/80

Internet

http://www.mdpi.com/1422-0067/18/1/80

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