Proteomics - a new and fast tool for identification of barley varieties.

• Main Authors: J. CHMELÍK, P. ŘEHULKA, C. MAYRHOFER, G. ALLMAIER
• Format: Article
• Language: ces
• Published: Research Institute of Brewing and Malting, Plc. 2001-06-01
• Series: Kvasný průmysl
• Subjects: proteomics; protein identification; barley; variety identification; mass spectrometry
• Online Access: https://kvasnyprumysl.cz/en/artkey/kpr-200106-0002_Proteomika_-_novy_a_rychly_nastroj_pro_identifikaci_odrud_jecmene.php

Identification of several proteins from various barley samples by the proteomic method based on combination of gel electrophoresis, MALDI-TOF mass spectrometry, and bioinformatics is an important tool for proteome analysis of barley and it can be successfully applied to the identification of malting barley varieties. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. The identified proteins were ribulose bishophate carboxylase from barley leaves and beta-amylase and aldose-reductase from barley grainsIdentification of several proteins from various barley samples by the proteomic method based on combination of gel electrophoresis, MALDI-TOF mass spectrometry, and bioinformatics is an important tool for proteome analysis of barley and it can be successfully applied to the identification of malting barley varieties. The mass spectrometry-based strategy designed for protein identification exhibits good sensitivity and rapid protein identification in comparison to other methods. The identified proteins were ribulose bishophate carboxylase from barley leaves and beta-amylase and aldose-reductase from barley grains