Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells in vitro

• Main Authors: Hrčková G., Mačák Kubašková T., Mudroňová D., Bardelčíková A.
• Format: Article
• Language: English
• Published: Sciendo 2020-11-01
• Series: Acta Facultatis Pharmaceuticae Universitatis Comenianae
• Subjects: silymarin; mouse; splenocytes; proliferation; mitochondrial potential; apoptosis
• Online Access: https://doi.org/10.2478/afpuc-2020-0003

Silymarin (SIL), a mixture of phenolic compounds, has a pleiotropic mode of action on various cell types, including immune cells. In this study, we investigated the concentration-dependent effect of SIL on proliferation of concanavalin A (CoA)-stimulated mouse spleen T lymphocytes, their viability, and secretion of IFN-g and IL-4 cytokines ex vivo in relation to gene expressions of transcription factors nuclear factor kappa B and Foxp3. In addition, metabolic activity of T cells was determined as changes in the mitochondrial membrane potential and apoptosis.