Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells in vitro

Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells in vitro

Silymarin (SIL), a mixture of phenolic compounds, has a pleiotropic mode of action on various cell types, including immune cells. In this study, we investigated the concentration-dependent effect of SIL on proliferation of concanavalin A (CoA)-stimulated mouse spleen T lymphocytes, their viability,...

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Bibliographic Details
Main Authors: Hrčková G., Mačák Kubašková T., Mudroňová D., Bardelčíková A.
Format: Article
Language:English
Published: Sciendo 2020-11-01
Series:Acta Facultatis Pharmaceuticae Universitatis Comenianae
Subjects:
silymarin
mouse
splenocytes
proliferation
mitochondrial potential
apoptosis
Online Access:https://doi.org/10.2478/afpuc-2020-0003
Description
Summary:Silymarin (SIL), a mixture of phenolic compounds, has a pleiotropic mode of action on various cell types, including immune cells. In this study, we investigated the concentration-dependent effect of SIL on proliferation of concanavalin A (CoA)-stimulated mouse spleen T lymphocytes, their viability, and secretion of IFN-g and IL-4 cytokines ex vivo in relation to gene expressions of transcription factors nuclear factor kappa B and Foxp3. In addition, metabolic activity of T cells was determined as changes in the mitochondrial membrane potential and apoptosis.
ISSN:1338-6786

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