The uptake of amino acids by lipids of pseudomonas aeruginosa*†

The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed...

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Main Authors:	R. Silberman, W.L. Gaby
Format:	Article
Language:	English
Published:	Elsevier 1961-04-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520390271

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spelling	doaj-879df83be8d94256baf08e39fa822e332021-04-23T06:10:53ZengElsevierJournal of Lipid Research0022-22751961-04-0122172176The uptake of amino acids by lipids of pseudomonas aeruginosa*†R. Silberman0W.L. Gaby1Department of Microbiology, Hahnemann Medical College and Hospital, Philadelphia 2, PennsylvaniaDepartment of Microbiology, Hahnemann Medical College and Hospital, Philadelphia 2, PennsylvaniaThe lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed at least six fractions. Two-dimensional chromatograms of the aqueous acid hydrolyzate of the phospholipids indicated the presence of at least 13 ninhydrin positive compounds. There was a correlation between the uptake of dl-alanine-1-C14, dl-leucine-1-C14, and dl-phenylalanine-3-Cl4 by phospholipids of the resting cells and utilization of these amino acids by the cell as indicated by manometric studies.http://www.sciencedirect.com/science/article/pii/S0022227520390271
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	R. Silberman W.L. Gaby
spellingShingle	R. Silberman W.L. Gaby The uptake of amino acids by lipids of pseudomonas aeruginosa*† Journal of Lipid Research
author_facet	R. Silberman W.L. Gaby
author_sort	R. Silberman
title	The uptake of amino acids by lipids of pseudomonas aeruginosa*†
title_short	The uptake of amino acids by lipids of pseudomonas aeruginosa*†
title_full	The uptake of amino acids by lipids of pseudomonas aeruginosa*†
title_fullStr	The uptake of amino acids by lipids of pseudomonas aeruginosa*†
title_full_unstemmed	The uptake of amino acids by lipids of pseudomonas aeruginosa*†
title_sort	uptake of amino acids by lipids of pseudomonas aeruginosa*†
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1961-04-01
description	The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed at least six fractions. Two-dimensional chromatograms of the aqueous acid hydrolyzate of the phospholipids indicated the presence of at least 13 ninhydrin positive compounds. There was a correlation between the uptake of dl-alanine-1-C14, dl-leucine-1-C14, and dl-phenylalanine-3-Cl4 by phospholipids of the resting cells and utilization of these amino acids by the cell as indicated by manometric studies.
url	http://www.sciencedirect.com/science/article/pii/S0022227520390271
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The uptake of amino acids by lipids of pseudomonas aeruginosa*†

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