The uptake of amino acids by lipids of pseudomonas aeruginosa*†
The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1961-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520390271 |
| Summary: | The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed at least six fractions. Two-dimensional chromatograms of the aqueous acid hydrolyzate of the phospholipids indicated the presence of at least 13 ninhydrin positive compounds. There was a correlation between the uptake of dl-alanine-1-C14, dl-leucine-1-C14, and dl-phenylalanine-3-Cl4 by phospholipids of the resting cells and utilization of these amino acids by the cell as indicated by manometric studies. |
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| ISSN: | 0022-2275 |