P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats

Purpose Evidence shows that adenosine triphosphate (ATP) is involved in the transmission of multiple chronic pain via P2X7 receptor. This study was to investigate the P2X7 and microglial cells in the chronic prostatitis pain. Materials and Methods Rats were divided into control group and chronic pro...

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Main Authors: Heng Zhang, Limei Liu, Zhong Yang, Jinhong Pan, Zhiwen Chen, Qiang Fang, Weibin Li, Longkun Li, Gengsheng Lu, Zhansong Zhou
Format: Article
Language:English
Published: Sociedade Brasileira de Urologia 2013-04-01
Series:International Brazilian Journal of Urology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382013000200276
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spelling doaj-878be755826442e3a6da66649a48b3232020-11-25T00:11:53ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-55381677-61192013-04-01392276285P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated ratsHeng ZhangLimei LiuZhong YangJinhong PanZhiwen ChenQiang FangWeibin LiLongkun LiGengsheng LuZhansong ZhouPurpose Evidence shows that adenosine triphosphate (ATP) is involved in the transmission of multiple chronic pain via P2X7 receptor. This study was to investigate the P2X7 and microglial cells in the chronic prostatitis pain. Materials and Methods Rats were divided into control group and chronic prostatitis group (n = 24 per group). A chronic prostatitis animal model was established by injecting complete Freund's adjuvant (CFA) to the prostate of rats, and the thermal withdrawal latency (TWL) was detected on days 0, 4, 12 and 24 (n = 6 at each time point in each group). Animals were sacrificed and the pathological examination of the prostate, detection of mRNA expression of P2X7 and ionized calcium binding adaptor molecule 1 (IBA-1) and measurement of content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the dorsal horn of L5-S2 spinal cord were performed on days 0, 4, 12 and 24. In addition, the content of TNF-α and IL-1β in the dorsal horn of L5-S2 spinal cord was measured after intrathecal injection of inhibitors of microglial cells and/or P2X7 for 5 days. Results The chronic prostatitis was confirmed by pathological examination. The expression of P2X7 and IBA-1 and the content of TNF-α and IL-1β in rats with chronic prostatitis were significantly higher than those in the control group. On day 4, the expressions of pro-inflammatory cytokines became to increase, reaching a maximal level on day 12 and started to reduce on day 24, but remained higher than that in the control group. Following suppression of microglial cells and P2X7 receptor, the secretion of TNF-α and IL-1β was markedly reduced. Conclusion In chronic prostatitis pain, the microglial cells and P2X7 receptor are activated resulting in the increased expression of TNF-α and IL-1β in the L5-S2 spinal cord, which might attribute to the maintenance and intensification of pain in chronic prostatitis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382013000200276ProstatitisCellsReceptors, Purinergic P2X7Tumor Necrosis Factor-alphaInterleukin-1
collection DOAJ
language English
format Article
sources DOAJ
author Heng Zhang
Limei Liu
Zhong Yang
Jinhong Pan
Zhiwen Chen
Qiang Fang
Weibin Li
Longkun Li
Gengsheng Lu
Zhansong Zhou
spellingShingle Heng Zhang
Limei Liu
Zhong Yang
Jinhong Pan
Zhiwen Chen
Qiang Fang
Weibin Li
Longkun Li
Gengsheng Lu
Zhansong Zhou
P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
International Brazilian Journal of Urology
Prostatitis
Cells
Receptors, Purinergic P2X7
Tumor Necrosis Factor-alpha
Interleukin-1
author_facet Heng Zhang
Limei Liu
Zhong Yang
Jinhong Pan
Zhiwen Chen
Qiang Fang
Weibin Li
Longkun Li
Gengsheng Lu
Zhansong Zhou
author_sort Heng Zhang
title P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
title_short P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
title_full P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
title_fullStr P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
title_full_unstemmed P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
title_sort p2x7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
publisher Sociedade Brasileira de Urologia
series International Brazilian Journal of Urology
issn 1677-5538
1677-6119
publishDate 2013-04-01
description Purpose Evidence shows that adenosine triphosphate (ATP) is involved in the transmission of multiple chronic pain via P2X7 receptor. This study was to investigate the P2X7 and microglial cells in the chronic prostatitis pain. Materials and Methods Rats were divided into control group and chronic prostatitis group (n = 24 per group). A chronic prostatitis animal model was established by injecting complete Freund's adjuvant (CFA) to the prostate of rats, and the thermal withdrawal latency (TWL) was detected on days 0, 4, 12 and 24 (n = 6 at each time point in each group). Animals were sacrificed and the pathological examination of the prostate, detection of mRNA expression of P2X7 and ionized calcium binding adaptor molecule 1 (IBA-1) and measurement of content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the dorsal horn of L5-S2 spinal cord were performed on days 0, 4, 12 and 24. In addition, the content of TNF-α and IL-1β in the dorsal horn of L5-S2 spinal cord was measured after intrathecal injection of inhibitors of microglial cells and/or P2X7 for 5 days. Results The chronic prostatitis was confirmed by pathological examination. The expression of P2X7 and IBA-1 and the content of TNF-α and IL-1β in rats with chronic prostatitis were significantly higher than those in the control group. On day 4, the expressions of pro-inflammatory cytokines became to increase, reaching a maximal level on day 12 and started to reduce on day 24, but remained higher than that in the control group. Following suppression of microglial cells and P2X7 receptor, the secretion of TNF-α and IL-1β was markedly reduced. Conclusion In chronic prostatitis pain, the microglial cells and P2X7 receptor are activated resulting in the increased expression of TNF-α and IL-1β in the L5-S2 spinal cord, which might attribute to the maintenance and intensification of pain in chronic prostatitis.
topic Prostatitis
Cells
Receptors, Purinergic P2X7
Tumor Necrosis Factor-alpha
Interleukin-1
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382013000200276
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