Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39

Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39

Ovarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).

Bibliographic Details
Main Authors: Deeti K. Shetty, Maneesha S. Inamdar
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506115002172
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spelling doaj-8789b707473046b490e7316e8d4a7fc22020-11-24T21:07:09ZengElsevierStem Cell Research1873-50611876-77532016-03-0116230831010.1016/j.scr.2015.12.037Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39Deeti K. Shetty0Maneesha S. Inamdar1Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, IndiaJawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, IndiaOvarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).http://www.sciencedirect.com/science/article/pii/S1873506115002172
collection DOAJ
language English
format Article
sources DOAJ
author Deeti K. Shetty
Maneesha S. Inamdar
spellingShingle Deeti K. Shetty
Maneesha S. Inamdar
Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39
Stem Cell Research
author_facet Deeti K. Shetty
Maneesha S. Inamdar
author_sort Deeti K. Shetty
title Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39
title_short Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39
title_full Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39
title_fullStr Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39
title_full_unstemmed Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39
title_sort generation of a heterozygous knockout human embryonic stem cell line for the ociad1 locus using crispr/cas9 mediated targeting: bjnhem20-ociad1-crispr-39
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description Ovarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).
url http://www.sciencedirect.com/science/article/pii/S1873506115002172
work_keys_str_mv AT deetikshetty generationofaheterozygousknockouthumanembryonicstemcelllinefortheociad1locususingcrisprcas9mediatedtargetingbjnhem20ociad1crispr39
AT maneeshasinamdar generationofaheterozygousknockouthumanembryonicstemcelllinefortheociad1locususingcrisprcas9mediatedtargetingbjnhem20ociad1crispr39
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