Genetic Variation of Apolipoprotein E (ApoE) in Surabaya, Palu and Alor Populations of Indonesia

• Main Authors: Pramudji Hastuti, Abdul Salam Mudzakir Sofro, Ahmad Husain Asdie, Ahmad Hamim Sadewa
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7843

Abstract<br />Apolipoprotein E (ApoE) has been considered to play an important role in cardiovascular disorders.<br />Several studies reported that genetic variation in ApoE locus influence plasma lipoprotein level. The objectives<br />of this study was to compare the frequency of ApoE genotypes and alleles in some populations of Indonesia.<br />One hundred and ninety five voluntarily unrelated apparently healthy individuals were recruited from<br />Surabaya, Palu and Alor representing the western, middle and eastern populations of Indonesia, respectively.<br />Blood samples were collected from each subject for DNA extraction. The common allelic variants of ApoE were<br />screened using polymerase chain reaction (PCR) and restriction fragment length polymorphism. Three alleles<br />i.e. &epsilon;2, &epsilon;3 and &epsilon;4 were identified and five genotypes i.e. ApoE &epsilon;2/&epsilon;2, ApoE &epsilon;2/&epsilon;3, ApoE &epsilon;3/&epsilon;3, ApoE &epsilon;2/&epsilon;4, ApoE &epsilon;3/<br />&epsilon;4 were found in three populations studied, while ApoE &epsilon;4/&epsilon;4 was absent in Surabaya, representing the western<br />populations of Indonesia. The frequency of &epsilon;2, &epsilon;3 and &epsilon;4 alleles in the western population were 0.208, 0.701<br />and 0.092 respectively; in the middle population were 0.242, 0.618 and 0.140 respectively and in the eastern<br />population of Indonesia were 0.267, 0.466 and 0.267 respectively. The highest frequency of &epsilon;2 and &epsilon;4 allele<br />was found in the eastern population of Indonesia. The distribution of &epsilon;2 allele were not significantly different<br />among all Indonesian populations, but significantly different were found in &epsilon;3 and &epsilon;4 allele in the eastern<br />population compared to those in the western and middle populations of Indonesian. It can be concluded that<br />the frequency of three ApoE alleles in the western and middle populations of Indonesia was not significantly<br />different however, significantly different was observed in the frequency of ApoE &epsilon;3 and &epsilon;4 alleles from the<br />eastern compared to those in the western and middle populations of Indonesia.<br />Keywords : Apolipoprotein E; genotypes; allele frequency; populations of Indonesia