MicroRNA-4728 serves as a suppressor and antagonist of oncogenic MAPK in Burkitt lymphoma

• Main Authors: Wei Wang, Peng Lin, Haiying Yao, Xi Jia, Jirui Sun, Lin Feng
• Format: Article
• Language: English
• Published: Elsevier 2018-07-01
• Series: Saudi Journal of Biological Sciences
• Online Access: http://www.sciencedirect.com/science/article/pii/S1319562X18301219

Objective: This study identified the biological role of miR-4728 in Burkitt lymphoma (BL) process. Methods: Ramos cells were used to analyze MicroRNA-4728 (miR-4728) biological functions. MiR-4728 expression was investigated in 14 randomly chosen tumor tissues and 12 noncancerous tissues by qRT-PCR. Cyquant assay was used to monitor cell proliferation. Colony formation assay was performed to study the effectiveness of miR-4728 on the proliferation of cells. The effects of miR-4728 on MAPK signaling pathway were detected by luciferase reporter assay. The significance of differences between groups were evaluated by SPSS. Results: In this study, MiRNA-4728 was observed to down-regulated in BL tissues compared to the noncancerous tissues. Additionally, miR-4728 inhibited Ramos cell proliferation. Moreover, miR-4728 overexpression also decreased the MAPK signaling activity. Conclusion: Our results suggested that miR-4728 serves as a suppressor and antagonist of oncogenic MAPK in Burkitt lymphoma. The appropriate regulation of miR-4728 might be vital to improve BL treatment. Keywords: miR-4728, MAPK, Burkitt lymphoma