Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amp...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Genética
2008-01-01
|
Series: | Genetics and Molecular Biology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027 |
id |
doaj-8751efa82ff947519c3c59605f89faef |
---|---|
record_format |
Article |
spelling |
doaj-8751efa82ff947519c3c59605f89faef2020-11-25T01:58:44ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852008-01-0131131732310.1590/S1415-47572008000200027Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban streamSilvia H. SofiaBruno A. GalindoFrancine M. PaulaLeda M.K. SodréCláudia B.R. MartinezIn this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784; p > 0.05), all others pairwise phiST values were significantly greater than zero, indicating moderate genetic differentiation among catfish samples from four localities. The relative low levels of genetic variation detected in all groups studied could be related to different factors, including the sedentary habit of these fish, which can be eroding the genetic variation of H. ancistroides from each locality.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027Loricariid fishgenetic diversityRAPDSiluriformes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silvia H. Sofia Bruno A. Galindo Francine M. Paula Leda M.K. Sodré Cláudia B.R. Martinez |
spellingShingle |
Silvia H. Sofia Bruno A. Galindo Francine M. Paula Leda M.K. Sodré Cláudia B.R. Martinez Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream Genetics and Molecular Biology Loricariid fish genetic diversity RAPD Siluriformes |
author_facet |
Silvia H. Sofia Bruno A. Galindo Francine M. Paula Leda M.K. Sodré Cláudia B.R. Martinez |
author_sort |
Silvia H. Sofia |
title |
Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream |
title_short |
Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream |
title_full |
Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream |
title_fullStr |
Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream |
title_full_unstemmed |
Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream |
title_sort |
genetic diversity of hypostomus ancistroides (teleostei, loricariidae) from an urban stream |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
2008-01-01 |
description |
In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784; p > 0.05), all others pairwise phiST values were significantly greater than zero, indicating moderate genetic differentiation among catfish samples from four localities. The relative low levels of genetic variation detected in all groups studied could be related to different factors, including the sedentary habit of these fish, which can be eroding the genetic variation of H. ancistroides from each locality. |
topic |
Loricariid fish genetic diversity RAPD Siluriformes |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027 |
work_keys_str_mv |
AT silviahsofia geneticdiversityofhypostomusancistroidesteleosteiloricariidaefromanurbanstream AT brunoagalindo geneticdiversityofhypostomusancistroidesteleosteiloricariidaefromanurbanstream AT francinempaula geneticdiversityofhypostomusancistroidesteleosteiloricariidaefromanurbanstream AT ledamksodre geneticdiversityofhypostomusancistroidesteleosteiloricariidaefromanurbanstream AT claudiabrmartinez geneticdiversityofhypostomusancistroidesteleosteiloricariidaefromanurbanstream |
_version_ |
1724968509451010048 |