Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream

In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amp...

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Main Authors: Silvia H. Sofia, Bruno A. Galindo, Francine M. Paula, Leda M.K. Sodré, Cláudia B.R. Martinez
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2008-01-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027
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spelling doaj-8751efa82ff947519c3c59605f89faef2020-11-25T01:58:44ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852008-01-0131131732310.1590/S1415-47572008000200027Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban streamSilvia H. SofiaBruno A. GalindoFrancine M. PaulaLeda M.K. SodréCláudia B.R. MartinezIn this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784; p > 0.05), all others pairwise phiST values were significantly greater than zero, indicating moderate genetic differentiation among catfish samples from four localities. The relative low levels of genetic variation detected in all groups studied could be related to different factors, including the sedentary habit of these fish, which can be eroding the genetic variation of H. ancistroides from each locality.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027Loricariid fishgenetic diversityRAPDSiluriformes
collection DOAJ
language English
format Article
sources DOAJ
author Silvia H. Sofia
Bruno A. Galindo
Francine M. Paula
Leda M.K. Sodré
Cláudia B.R. Martinez
spellingShingle Silvia H. Sofia
Bruno A. Galindo
Francine M. Paula
Leda M.K. Sodré
Cláudia B.R. Martinez
Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
Genetics and Molecular Biology
Loricariid fish
genetic diversity
RAPD
Siluriformes
author_facet Silvia H. Sofia
Bruno A. Galindo
Francine M. Paula
Leda M.K. Sodré
Cláudia B.R. Martinez
author_sort Silvia H. Sofia
title Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
title_short Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
title_full Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
title_fullStr Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
title_full_unstemmed Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
title_sort genetic diversity of hypostomus ancistroides (teleostei, loricariidae) from an urban stream
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2008-01-01
description In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784; p > 0.05), all others pairwise phiST values were significantly greater than zero, indicating moderate genetic differentiation among catfish samples from four localities. The relative low levels of genetic variation detected in all groups studied could be related to different factors, including the sedentary habit of these fish, which can be eroding the genetic variation of H. ancistroides from each locality.
topic Loricariid fish
genetic diversity
RAPD
Siluriformes
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027
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