Microsatellite Primers in the Foundation Tree Species <i>Pinus edulis</i> and <i>P. monophylla</i> (Pinaceae)

• Main Authors: Andrew L. Krohn, Lluvia Flores-Rentería, Catherine A. Gehring
• Format: Article
• Language: English
• Published: Wiley 2013-07-01
• Series: Applications in Plant Sciences
• Subjects: microsatellite; Pinaceae; Pinus edulis; Pinus monophylla; population genetics
• Online Access: http://www.bioone.org/doi/full/10.3732/apps.1200552

Premise of the study: Microsatellite primers were developed in the foundational tree species <i>Pinus edulis</i> to investigate population differentiation of <i>P. edulis</i> and hybridization among closely related species. Methods and Results: Using a hybridization protocol, primer sets for 11 microsatellite loci were developed using megagametophyte tissue from <i>P. edulis</i> and scored for polymorphism in three populations of <i>P. edulis</i> and a single <i>P. monophylla</i> population. The primers amplified simple and compound di-, tri-, and pentanucleotide repeats with two to 18 alleles per locus. Conclusions: These results demonstrate the utility of the described primers for studies of population differentiation within and among <i>P. edulis</i> populations as well as across putative hybrid zones where <i>P. edulis</i> may coexist with sister species.