Analysis of case-parent trios at a locus with a deletion allele: association of GSTM1 with autism

• Main Authors: Wang Rong, Ming Sue X, Stenroos Edward S, Mars Audrey E, Williams Tanishia A, Buyske Steven, Sreenath Madhura, Factura Marivic F, Reddy Chitra, Lambert George H, Johnson William G
• Format: Article
• Language: English
• Published: BMC 2006-02-01
• Series: BMC Genetics
• Online Access: http://www.biomedcentral.com/1471-2156/7/8

<p>Abstract</p> <p>Background</p> <p>Certain loci on the human genome, such as glutathione S-transferase M1 (GSTM1), do not permit heterozygotes to be reliably determined by commonly used methods. Association of such a locus with a disease is therefore generally tested with a case-control design. When subjects have already been ascertained in a case-parent design however, the question arises as to whether the data can still be used to test disease association at such a locus.</p> <p>Results</p> <p>A likelihood ratio test was constructed that can be used with a case-parents design but has somewhat less power than a Pearson's chi-squared test that uses a case-control design. The test is illustrated on a novel dataset showing a genotype relative risk near 2 for the homozygous GSTM1 deletion genotype and autism.</p> <p>Conclusion</p> <p>Although the case-control design will remain the mainstay for a locus with a deletion, the likelihood ratio test will be useful for such a locus analyzed as part of a larger case-parent study design. The likelihood ratio test has the advantage that it can incorporate complete and incomplete case-parent trios as well as independent cases and controls. Both analyses support (<it>p </it>= 0.046 for the proposed test, <it>p </it>= 0.028 for the case-control analysis) an association of the homozygous GSTM1 deletion genotype with autism.</p>