| Summary: | The design principle of a biochemiluminescent instrument is presented. The instrument diagnoses influenza based on detection of the flu viral neuraminidase activity. The instrument uses a photomultiplier tube (PMT) as sensor, with 24 test channels. The reaction disk is driven by step motor to rotate, where the reagent is incubated. Finally, PMT detects the number of photons emitted by the reagent and calculates the enzyme activity. The correlation coefficient (R2) of 2.83 to 5.06 log TCID50/ml influenza virus was 0.9967; the detection limits of the two positive virus samples were TCID50/mL and 953 TCID50/mL, respectively, The positive predictive value (PPV) was 100%, and the negative predictive value (NPV) was 98.90% in clinical evaluation. According to the evaluation, the designed biochemiluminescent instrument can well match with the reagents, and meet with the performance requirements of clinical setting.
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