| Summary: | The endogenous content of IAA in the cotyledons of Pharbitis nil is low before and during the first half of the inductive 16-h-long dark period. From the 8th to the 12th hour the level of IAA increased and then again was going down at the end of a dark period. Exogenous IAA applied to the cotyledones before and during the first half of the inductive dark period inhibits flower bud formation. The application of IAA to shoot apex also resulted in the inhibition of flowering. Experiments with TIBA, an auxin polar transport inhibitor, and PCIB, an auxin action inhibitor, have shown that auxin polar transport in cotyledones and long-distance auxin transport from cotyledones to shoot apex play an important role in IAA inhibition of flower bud formation. It suggests that auxins play their role not only at the level of floral induction in cotyledones, but also in the later events of floral evocation and differentiation in shoot apex.
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