Characterization of Xylanase Streptomyces spp. SKK1-8

Characterization of Xylanase Streptomyces spp. SKK1-8

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Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 °C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 °C for 48 hou...

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Main Authors: ANJA MERYANDINI, TRIO HENDARWIN, DEDEN SAPRUDIN, YULIN LESTARI
Format: Article
Language:English
Published: Bogor Agricultural University 2006-12-01
Series:Hayati Journal of Biosciences
Subjects:
xilanase
Streptomyces spp.
characterization
zymogram and SDS-PAGE
stability
Online Access:http://www.sciencedirect.com/science/article/pii/S1978301916303102
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spelling doaj-86b68c6504524213931e7236f4f0c7802020-11-24T21:07:28ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192006-12-0113415115510.1016/S1978-3019(16)30310-2Characterization of Xylanase Streptomyces spp. SKK1-8ANJA MERYANDINI0TRIO HENDARWIN1DEDEN SAPRUDIN2YULIN LESTARI3Departemen Biologi, FMIPA, Institut Pertanian Bogor, Jalan Raya Pajajaran. Bogor 16144Departemen Biologi, FMIPA, Institut Pertanian Bogor, Jalan Raya Pajajaran. Bogor 16144Departemen Kimia, FMIPA, Institut Pertanian Bogor, Jalan Raya Pajajaran, Bogor 16144Departemen Biologi, FMIPA, Institut Pertanian Bogor, Jalan Raya Pajajaran. Bogor 16144Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 °C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 °C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 °C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan.http://www.sciencedirect.com/science/article/pii/S1978301916303102xilanaseStreptomyces spp.characterizationzymogram and SDS-PAGEstability
collection DOAJ
language English
format Article
sources DOAJ
author ANJA MERYANDINI
TRIO HENDARWIN
DEDEN SAPRUDIN
YULIN LESTARI
spellingShingle ANJA MERYANDINI
TRIO HENDARWIN
DEDEN SAPRUDIN
YULIN LESTARI
Characterization of Xylanase Streptomyces spp. SKK1-8
Hayati Journal of Biosciences
xilanase
Streptomyces spp.
characterization
zymogram and SDS-PAGE
stability
author_facet ANJA MERYANDINI
TRIO HENDARWIN
DEDEN SAPRUDIN
YULIN LESTARI
author_sort ANJA MERYANDINI
title Characterization of Xylanase Streptomyces spp. SKK1-8
title_short Characterization of Xylanase Streptomyces spp. SKK1-8
title_full Characterization of Xylanase Streptomyces spp. SKK1-8
title_fullStr Characterization of Xylanase Streptomyces spp. SKK1-8
title_full_unstemmed Characterization of Xylanase Streptomyces spp. SKK1-8
title_sort characterization of xylanase streptomyces spp. skk1-8
publisher Bogor Agricultural University
series Hayati Journal of Biosciences
issn 1978-3019
publishDate 2006-12-01
description Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 °C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 °C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 °C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan.
topic xilanase
Streptomyces spp.
characterization
zymogram and SDS-PAGE
stability
url http://www.sciencedirect.com/science/article/pii/S1978301916303102
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AT triohendarwin characterizationofxylanasestreptomycessppskk18
AT dedensaprudin characterizationofxylanasestreptomycessppskk18
AT yulinlestari characterizationofxylanasestreptomycessppskk18
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