Characterization of Xylanase Streptomyces spp. SKK1-8

Characterization of Xylanase Streptomyces spp. SKK1-8

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Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 °C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 °C for 48 hou...

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Bibliographic Details
Main Authors: ANJA MERYANDINI, TRIO HENDARWIN, DEDEN SAPRUDIN, YULIN LESTARI
Format: Article
Language:English
Published: Bogor Agricultural University 2006-12-01
Series:Hayati Journal of Biosciences
Subjects:
xilanase
Streptomyces spp.
characterization
zymogram and SDS-PAGE
stability
Online Access:http://www.sciencedirect.com/science/article/pii/S1978301916303102
Description
Summary:Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 °C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 °C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 °C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan.
ISSN:1978-3019

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