Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves
Chlorophyllide (chlide) is a natural catabolic product of chlorophyll (Chl), produced through the activity of chlorophyllase (chlase). The growth inhibitory and antioxidant effects of chlide from different plant leaf extracts have not been reported. The aim of this study is to demonstrate that chlid...
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doaj-86b03a11631c4dac85020ce45da9d4182020-11-25T01:29:35ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882019-01-01201910.1155/2019/94943289494328Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant LeavesYi-Ting Wang0Chih-Hui Yang1Ting-Yu Huang2Mi‐Hsueh Tai3Ru-Han Sie4Jei-Fu Shaw5Department of Biological Science and Technology, I-Shou University, TaiwanDepartment of Biological Science and Technology, I-Shou University, TaiwanDepartment of Biological Science and Technology, I-Shou University, TaiwanDepartment of Biological Science and Technology, I-Shou University, TaiwanDepartment of Biological Science and Technology, I-Shou University, TaiwanDepartment of Biological Science and Technology, I-Shou University, TaiwanChlorophyllide (chlide) is a natural catabolic product of chlorophyll (Chl), produced through the activity of chlorophyllase (chlase). The growth inhibitory and antioxidant effects of chlide from different plant leaf extracts have not been reported. The aim of this study is to demonstrate that chlide in crude extracts from leaves has the potential to exert cytotoxic effects on cancer cell lines. The potential inhibitory and antioxidant effects of chlide in crude extracts from 10 plant leaves on breast cancer cells (MCF7 and MDA-MB-231), hepatocellular carcinoma cells (Hep G2), colorectal adenocarcinoma cells (Caco2), and glioblastoma cells (U-118 MG) were studied using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. The results of the MTT assay showed that chlide in crude extracts from sweet potato were the most effective against all cancer cell lines tested. U-118 MG cells were the most sensitive, while Caco2 cells were the most resistant to the tested crude extracts. The cytotoxic effects of chlide and Chl in crude extracts from sweet potato and of commercial chlorophyllin (Cu-chlin), in descending order, were as follows: chlide > Chl > Cu-chlin. Notably, the IC50 of sweet potato in U-118 MG cells was 45.65 μg/mL while those of Chl and Cu-chlin exceeded 200 μg/mL. In the DPPH assay, low concentrations (100 μg/mL) of chlide and Cu-chlin from crude extracts of sweet potato presented very similar radical scavenging activity to vitamin B2. The concentration of chlide was negatively correlated with DPPH activity. The current study was the first to demonstrate that chlide in crude extracts from leaves have potential cytotoxicity in cancer cell lines. Synergism between chlide and other compounds from leaf crude extracts may contribute to its cytotoxicity.http://dx.doi.org/10.1155/2019/9494328 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi-Ting Wang Chih-Hui Yang Ting-Yu Huang Mi‐Hsueh Tai Ru-Han Sie Jei-Fu Shaw |
spellingShingle |
Yi-Ting Wang Chih-Hui Yang Ting-Yu Huang Mi‐Hsueh Tai Ru-Han Sie Jei-Fu Shaw Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves Evidence-Based Complementary and Alternative Medicine |
author_facet |
Yi-Ting Wang Chih-Hui Yang Ting-Yu Huang Mi‐Hsueh Tai Ru-Han Sie Jei-Fu Shaw |
author_sort |
Yi-Ting Wang |
title |
Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves |
title_short |
Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves |
title_full |
Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves |
title_fullStr |
Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves |
title_full_unstemmed |
Cytotoxic Effects of Chlorophyllides in Ethanol Crude Extracts from Plant Leaves |
title_sort |
cytotoxic effects of chlorophyllides in ethanol crude extracts from plant leaves |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2019-01-01 |
description |
Chlorophyllide (chlide) is a natural catabolic product of chlorophyll (Chl), produced through the activity of chlorophyllase (chlase). The growth inhibitory and antioxidant effects of chlide from different plant leaf extracts have not been reported. The aim of this study is to demonstrate that chlide in crude extracts from leaves has the potential to exert cytotoxic effects on cancer cell lines. The potential inhibitory and antioxidant effects of chlide in crude extracts from 10 plant leaves on breast cancer cells (MCF7 and MDA-MB-231), hepatocellular carcinoma cells (Hep G2), colorectal adenocarcinoma cells (Caco2), and glioblastoma cells (U-118 MG) were studied using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. The results of the MTT assay showed that chlide in crude extracts from sweet potato were the most effective against all cancer cell lines tested. U-118 MG cells were the most sensitive, while Caco2 cells were the most resistant to the tested crude extracts. The cytotoxic effects of chlide and Chl in crude extracts from sweet potato and of commercial chlorophyllin (Cu-chlin), in descending order, were as follows: chlide > Chl > Cu-chlin. Notably, the IC50 of sweet potato in U-118 MG cells was 45.65 μg/mL while those of Chl and Cu-chlin exceeded 200 μg/mL. In the DPPH assay, low concentrations (100 μg/mL) of chlide and Cu-chlin from crude extracts of sweet potato presented very similar radical scavenging activity to vitamin B2. The concentration of chlide was negatively correlated with DPPH activity. The current study was the first to demonstrate that chlide in crude extracts from leaves have potential cytotoxicity in cancer cell lines. Synergism between chlide and other compounds from leaf crude extracts may contribute to its cytotoxicity. |
url |
http://dx.doi.org/10.1155/2019/9494328 |
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