A High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations

A High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations

In crop research programs that implement transgene-based strategies for trait improvement it is necessary to distinguish between transgene homozygous and hemizygous individuals in segregating populations. Direct methods for determining transgene zygosity are technically challenging, expensive, and r...

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Bibliographic Details
Main Authors: Lige Geng, Dewayne D. Deng, Martin J. Wubben, Johnie N. Jenkins, Jack C. McCarty, Ibrokhim Abdurakhmonov
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-07-01
Series:Frontiers in Plant Science
Subjects:
transgene
PCR
bimodal
method
plant breeding
cotton
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01252/full

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