Optimisation of methods for quantifying plasma mRNA levels from genes responsible for coronary artery plaque development and destabilization

Optimisation of methods for quantifying plasma mRNA levels from genes responsible for coronary artery plaque development and destabilization

Aim To investigate the hypothesis that in patients with coronaryatherosclerosis it is possible to measure plasma mRNA levels fromgenes responsible for plaque development and destabilization.Methods Methods for RNA isolation, mRNA transcription and quantitative PCR were evaluated and optimised, in or...

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Bibliographic Details
Main Authors: Darko Černe, Irma Štern, Igor Kranjec, Janja Marc
Format: Article
Language:English
Published: Medical Association of Zenica-Doboj Canton 2011-02-01
Series:Medicinski Glasnik
Subjects:
plasma mRNA
coronary atherosclerosis
method optimisation
Online Access:http://www.ljkzedo.com.ba/medglasnik/vol81/MGvol81.2011_19.pdf

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http://www.ljkzedo.com.ba/medglasnik/vol81/MGvol81.2011_19.pdf

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