Screening of new secretory cellulases from different supernatants of white rot fungi from Misiones, Argentina

• Main Authors: Romina Olga Coniglio, María Isabel Fonseca, Laura Lidia Villalba, Pedro Darío Zapata
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2017-01-01
• Series: Mycology
• Subjects: Endoglucanase; cellobiohydrolase; β-glucosidase; white rot fungi; screening
• Online Access: http://dx.doi.org/10.1080/21501203.2016.1267047

Cellulases hydrolyse the cellulose chain into single sugars efficiently. These sugars can be fermented in the bioethanol process, a source of renewable energy. Misiones rainforest is one of the most biodiverse systems on the planet subtropical ecoregions, which is the most probable site to find new fungal strains with potential for degrading cellulose through cellulases. The aim of this work was to find an efficient cellulolytic microorganism through the exploration of native white rot fungi from Misiones. From the qualitative screening 11 fungal strains were selected. The quantitative analysis revealed that the isolated LBM 033 was the best cellulases producer, reaching 57, 226 and 387 U/l of cellobiohydrolase, β-glucosidase and endoglucanase activity, respectively. The zymograms showed that the molecular mass of most of the endoglucanases ranged from 69 to 88 kDa and the molecular mass of most of the cellobiohydrolases was 45 kDa. The search of new cellulases of secretory organisms should lead to an efficient degradation of cellulosic materials, and thus facilitating potential applications in the production of bioenergy from lignocellulosic biomass.