Multi locus sequence typing of <it>Chlamydiales</it>: clonal groupings within the obligate intracellular bacteria <it>Chlamydia trachomatis</it>

• Main Authors: Langerak Ankie A, Ossewaarde Jacobus M, Morré Servaas A, Kusecek Barica, Morelli Giovanna, Pannekoek Yvonne, van der Ende Arie
• Format: Article
• Language: English
• Published: BMC 2008-02-01
• Series: BMC Microbiology
• Online Access: http://www.biomedcentral.com/1471-2180/8/42

<p>Abstract</p> <p>Background</p> <p>The obligate intracellular growing bacterium <it>Chlamydia trachomatis </it>causes diseases like trachoma, urogenital infection and lymphogranuloma venereum with severe morbidity. Several serovars and genotypes have been identified, but these could not be linked to clinical disease or outcome. The related <it>Chlamydophila pneumoniae</it>, of which no subtypes are recognized, causes respiratory infections worldwide. We developed a multi locus sequence typing (MLST) scheme to understand the population genetic structure and diversity of these species and to evaluate the association between genotype and disease.</p> <p>Results</p> <p>A collection of 26 strains of <it>C. trachomatis </it>of different serovars and clinical presentation and 18 strains of <it>C. pneumoniae </it>were included in the study. For comparison, sequences of <it>C. abortus, C. psittaci</it>, <it>C. caviae</it>, <it>C. felis</it>, <it>C. pecorum </it>(<it>Chlamydophila</it>), <it>C. muridarum </it>(<it>Chlamydia</it>) and of <it>Candidatus protochlamydia </it>and <it>Simkania negevensis </it>were also included. Sequences of fragments (400 – 500 base pairs) from seven housekeeping genes (<it>enoA</it>, <it>fumC</it>, <it>gatA</it>, <it>gidA</it>, <it>hemN</it>, <it>hlfX</it>, <it>oppA</it>) were analysed. Analysis of allelic profiles by eBurst revealed three non-overlapping clonal complexes among the <it>C. trachomatis </it>strains, while the <it>C. pneumoniae </it>strains formed a single group. An UPGMA tree produced from the allelic profiles resulted in three groups of sequence types. The LGV strains grouped in a single cluster, while the urogenital strains were distributed over two separated groups, one consisted solely of strains with frequent occurring serovars (E, D and F). The distribution of the different serovars over the three groups was not consistent, suggesting exchange of serovar encoding <it>ompA </it>sequences. In one instance, exchange of <it>fumC </it>sequences between strains of different groups was observed. Cluster analyses of concatenated sequences of the Chlamydophila and Chlamydia species together with those of <it>Candidatus Protochlamydia amoebophila </it>and <it>Simkania negevensis </it>resulted in a tree identical to that obtained with 23S RNA gene sequences.</p> <p>Conclusion</p> <p>These data show that <it>C. trachomatis </it>and <it>C. pneumoniae </it>are highly uniform. The difference in genetic diversity between <it>C. trachomatis </it>and <it>C. pneumoniae </it>is in concordance with a later assimilation to the human host of the latter. Our data supports the taxonomy of the order of <it>Chlamydiales</it>.</p>