One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster

One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster

Summary: This one-step method generates, for any locus, a conditional knockout allele in Drosophila. The allele carries a bright fluorescent marker for easy screening and an attP landing site for subsequent genetic manipulations. After removing the selectable marker with Cre, the attP site can be us...

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Bibliographic Details
Main Authors:	Joyce J.S. Yu, Jean-Paul Vincent, Ian J. McGough
Format:	Article
Language:	English
Published:	Elsevier 2021-06-01
Series:	STAR Protocols
Subjects:	CRISPR Genetics Model Organisms
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721002677

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