Multiplexed CRISPR/Cas9 genome editing increases the efficacy of homologous-dependent repair of donor sequences in mammalian cells

Multiplexed CRISPR/Cas9 genome editing increases the efficacy of homologous-dependent repair of donor sequences in mammalian cells

Efficient and robust genome editing tools and strategies allow for specific and exact genetic changes to be captured in model systems, thereby accelerating both forward and reverse genetics studies. The development of CRISPR/Cas9 as a facile designer nuclease toolset has allowed for defined genetic...

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Bibliographic Details
Main Authors: Ezio T. Fok, Clement B. Penny, Musa M. Mhlanga, Marc S. Weinberg
Format: Article
Language:English
Published: Academy of Science of South Africa 2015-07-01
Series:South African Journal of Science
Subjects:
genome repair
genome targeting
sgRNA
HDR
T7 endonuclease I
Online Access:https://www.sajs.co.za/article/view/3708

Internet

https://www.sajs.co.za/article/view/3708

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