CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we utilized CRISPR/Cas9 to generate mammalian cell lines that express trunca...

Full description

Bibliographic Details
Main Authors: Kevin M. Bloh, Pawel A. Bialk, Anilkumar Gopalakrishnapillai, E. Anders Kolb, Eric B. Kmiec
Format: Article
Language:English
Published: Elsevier 2017-06-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
gene editing
Gata1s protein
acute myeloid leukemia in down syndrome
CRISPR/Cas9
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117301592

Similar Items

Similar Items