STUDY ON GERMPLASMIC RESOURCES OF LYCORIS LONGITUBA USING RAPD AND ISSR
The perianth DNA extraction methods were discussed as Lycoris longituba for example. By means of RAPD and ISSR, germplasmic resources of Lycoris longituba were primarily studied. The results were as follow: by RAPD, a total of 77 discernible loci were obtained using 12 primers, of which 53 loci we...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
"Alexandru Ioan Cuza" University of Iași
2006-08-01
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| Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
| Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/324 |
| Summary: | The perianth DNA extraction methods were discussed as Lycoris longituba for example. By means of RAPD
and ISSR, germplasmic resources of Lycoris longituba were primarily studied. The results were as follow: by RAPD, a
total of 77 discernible loci were obtained using 12 primers, of which 53 loci were polymorphic (PPB = 68.8%); by ISSR,
67 discernible loci were got using 9 primers, of which 62 loci were polymorphic (PPB = 92.5%). So, genetic diversity of
Lycoris longituba was abundant, whose germplasmic resources could be stored for breeding. From UPGMA dendrogram
of Lycoris longituba using RAPD or ISSR method, three Lycoris longituba types were supported with molecular evidence,
which were originally distinguished by flower color. Therefore, in the future use of Lycoris longituba germplasmic
resources, different varieties of Lycoris longituba could be cultivated. |
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| ISSN: | 1582-3571 2248-3276 |