A Tet-on system for DRD1-expressing cells.

A Tet-on system for DRD1-expressing cells.

Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of t...

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Main Authors: Fayi Yao, Paul D Walker, Robert G MacKenzie
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3742517?pdf=render
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spelling doaj-85e788bd86c3413d867eb66a36105cec2020-11-25T01:52:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7268110.1371/journal.pone.0072681A Tet-on system for DRD1-expressing cells.Fayi YaoPaul D WalkerRobert G MacKenzieCells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson's disease and addiction.http://europepmc.org/articles/PMC3742517?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Fayi Yao
Paul D Walker
Robert G MacKenzie
spellingShingle Fayi Yao
Paul D Walker
Robert G MacKenzie
A Tet-on system for DRD1-expressing cells.
PLoS ONE
author_facet Fayi Yao
Paul D Walker
Robert G MacKenzie
author_sort Fayi Yao
title A Tet-on system for DRD1-expressing cells.
title_short A Tet-on system for DRD1-expressing cells.
title_full A Tet-on system for DRD1-expressing cells.
title_fullStr A Tet-on system for DRD1-expressing cells.
title_full_unstemmed A Tet-on system for DRD1-expressing cells.
title_sort tet-on system for drd1-expressing cells.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson's disease and addiction.
url http://europepmc.org/articles/PMC3742517?pdf=render
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AT pauldwalker atetonsystemfordrd1expressingcells
AT robertgmackenzie atetonsystemfordrd1expressingcells
AT fayiyao tetonsystemfordrd1expressingcells
AT pauldwalker tetonsystemfordrd1expressingcells
AT robertgmackenzie tetonsystemfordrd1expressingcells
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