Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh

Prokaryotic organisms often react instantly to environmental variations to ensure their survival. They can achieve this by rapidly and specifically modulating translation, the critical step of protein synthesis. The translation machinery responds to an array of cis-acting elements, located on the RN...

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Main Authors: Marie-Claude Carrier, Evelyne Ng Kwan Lim, Gabriel Jeannotte, Eric Massé
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.609237/full
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spelling doaj-85e3a2a0a575463da82c4d93809a45882020-12-15T04:32:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-12-011110.3389/fmicb.2020.609237609237Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory MeshMarie-Claude CarrierEvelyne Ng Kwan LimGabriel JeannotteEric MasséProkaryotic organisms often react instantly to environmental variations to ensure their survival. They can achieve this by rapidly and specifically modulating translation, the critical step of protein synthesis. The translation machinery responds to an array of cis-acting elements, located on the RNA transcript, which dictate the fate of mRNAs. These cis-encoded elements, such as RNA structures or sequence motifs, interact with a variety of regulators, among them small regulatory RNAs. These small regulatory RNAs (sRNAs) are especially effective at modulating translation initiation through their interaction with cis-encoded mRNA elements. Here, through selected examples of canonical and non-canonical regulatory events, we demonstrate the intimate connection between mRNA cis-encoded features and sRNA-dependent translation regulation. We also address how sRNA-based mechanistic studies can drive the discovery of new roles for cis-elements. Finally, we briefly overview the challenges of using translation regulation by synthetic regulators as a tool.https://www.frontiersin.org/articles/10.3389/fmicb.2020.609237/fulltranslational regulationtranslational determinantscis regulatory elementssmall regulatory RNAsregulatory mechanismstranslational enhancers
collection DOAJ
language English
format Article
sources DOAJ
author Marie-Claude Carrier
Evelyne Ng Kwan Lim
Gabriel Jeannotte
Eric Massé
spellingShingle Marie-Claude Carrier
Evelyne Ng Kwan Lim
Gabriel Jeannotte
Eric Massé
Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh
Frontiers in Microbiology
translational regulation
translational determinants
cis regulatory elements
small regulatory RNAs
regulatory mechanisms
translational enhancers
author_facet Marie-Claude Carrier
Evelyne Ng Kwan Lim
Gabriel Jeannotte
Eric Massé
author_sort Marie-Claude Carrier
title Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh
title_short Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh
title_full Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh
title_fullStr Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh
title_full_unstemmed Trans-Acting Effectors Versus RNA Cis-Elements: A Tightly Knit Regulatory Mesh
title_sort trans-acting effectors versus rna cis-elements: a tightly knit regulatory mesh
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2020-12-01
description Prokaryotic organisms often react instantly to environmental variations to ensure their survival. They can achieve this by rapidly and specifically modulating translation, the critical step of protein synthesis. The translation machinery responds to an array of cis-acting elements, located on the RNA transcript, which dictate the fate of mRNAs. These cis-encoded elements, such as RNA structures or sequence motifs, interact with a variety of regulators, among them small regulatory RNAs. These small regulatory RNAs (sRNAs) are especially effective at modulating translation initiation through their interaction with cis-encoded mRNA elements. Here, through selected examples of canonical and non-canonical regulatory events, we demonstrate the intimate connection between mRNA cis-encoded features and sRNA-dependent translation regulation. We also address how sRNA-based mechanistic studies can drive the discovery of new roles for cis-elements. Finally, we briefly overview the challenges of using translation regulation by synthetic regulators as a tool.
topic translational regulation
translational determinants
cis regulatory elements
small regulatory RNAs
regulatory mechanisms
translational enhancers
url https://www.frontiersin.org/articles/10.3389/fmicb.2020.609237/full
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