A method to screen and evaluate tissue adhesives for joint repair applications

<p>Abstract</p> <p>Background</p> <p>Tissue adhesives are useful means for various medical procedures. Since varying requirements cause that a single adhesive cannot meet all needs, bond strength testing remains one of the key applications used to screen for new product...

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Main Authors: Dehne Tilo, Zehbe Rolf, Krüger Jan, Petrova Aneliya, Valbuena Rafael, Sittinger Michael, Schubert Helmut, Ringe Jochen
Format: Article
Language:English
Published: BMC 2012-09-01
Series:BMC Musculoskeletal Disorders
Subjects:
Online Access:http://www.biomedcentral.com/1471-2474/13/175
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spelling doaj-85bdd4ee0be74ea5be3bb7f9cf7c0ed82020-11-24T21:11:25ZengBMCBMC Musculoskeletal Disorders1471-24742012-09-0113117510.1186/1471-2474-13-175A method to screen and evaluate tissue adhesives for joint repair applicationsDehne TiloZehbe RolfKrüger JanPetrova AneliyaValbuena RafaelSittinger MichaelSchubert HelmutRinge Jochen<p>Abstract</p> <p>Background</p> <p>Tissue adhesives are useful means for various medical procedures. Since varying requirements cause that a single adhesive cannot meet all needs, bond strength testing remains one of the key applications used to screen for new products and study the influence of experimental variables. This study was conducted to develop an easy to use method to screen and evaluate tissue adhesives for tissue engineering applications.</p> <p>Method</p> <p>Tissue grips were designed to facilitate the reproducible production of substrate tissue and adhesive strength measurements in universal testing machines. Porcine femoral condyles were used to generate osteochondral test tissue cylinders (substrates) of different shapes. Viability of substrates was tested using PI/FDA staining. Self-bonding properties were determined to examine reusability of substrates (n = 3). Serial measurements (n = 5) in different operation modes (OM) were performed to analyze the bonding strength of tissue adhesives in bone (OM-1) and cartilage tissue either in isolation (OM-2) or under specific requirements in joint repair such as filling cartilage defects with clinical applied fibrin/PLGA-cell-transplants (OM-3) or tissues (OM-4). The efficiency of the method was determined on the basis of adhesive properties of fibrin glue for different assembly times (30 s, 60 s). Seven randomly generated collagen formulations were analyzed to examine the potential of method to identify new tissue adhesives.</p> <p>Results</p> <p>Viability analysis of test tissue cylinders revealed vital cells (>80%) in cartilage components even 48 h post preparation. Reuse (n = 10) of test substrate did not significantly change adhesive characteristics. Adhesive strength of fibrin varied in different test settings (OM-1: 7.1 kPa, OM-2: 2.6 kPa, OM-3: 32.7 kPa, OM-4: 30.1 kPa) and was increasing with assembly time on average (2.4-fold). The screening of the different collagen formulations revealed a substance with significant higher adhesive strength on cartilage (14.8 kPa) and bone tissue (11.8 kPa) compared to fibrin and also considerable adhesive properties when filling defects with cartilage tissue (23.2 kPa).</p> <p>Conclusion</p> <p>The method confirmed adhesive properties of fibrin and demonstrated the dependence of adhesive properties and applied settings. Furthermore the method was suitable to screen for potential adhesives and to identify a promising candidate for cartilage and bone applications. The method can offer simple, replicable and efficient evaluation of adhesive properties in <it>ex vivo</it> specimens and may be a useful supplement to existing methods in clinical relevant settings.</p> http://www.biomedcentral.com/1471-2474/13/175Tissue adhesiveBonding strengthCartilageBoneTransplantTissue engineeringJoint repairTest method
collection DOAJ
language English
format Article
sources DOAJ
author Dehne Tilo
Zehbe Rolf
Krüger Jan
Petrova Aneliya
Valbuena Rafael
Sittinger Michael
Schubert Helmut
Ringe Jochen
spellingShingle Dehne Tilo
Zehbe Rolf
Krüger Jan
Petrova Aneliya
Valbuena Rafael
Sittinger Michael
Schubert Helmut
Ringe Jochen
A method to screen and evaluate tissue adhesives for joint repair applications
BMC Musculoskeletal Disorders
Tissue adhesive
Bonding strength
Cartilage
Bone
Transplant
Tissue engineering
Joint repair
Test method
author_facet Dehne Tilo
Zehbe Rolf
Krüger Jan
Petrova Aneliya
Valbuena Rafael
Sittinger Michael
Schubert Helmut
Ringe Jochen
author_sort Dehne Tilo
title A method to screen and evaluate tissue adhesives for joint repair applications
title_short A method to screen and evaluate tissue adhesives for joint repair applications
title_full A method to screen and evaluate tissue adhesives for joint repair applications
title_fullStr A method to screen and evaluate tissue adhesives for joint repair applications
title_full_unstemmed A method to screen and evaluate tissue adhesives for joint repair applications
title_sort method to screen and evaluate tissue adhesives for joint repair applications
publisher BMC
series BMC Musculoskeletal Disorders
issn 1471-2474
publishDate 2012-09-01
description <p>Abstract</p> <p>Background</p> <p>Tissue adhesives are useful means for various medical procedures. Since varying requirements cause that a single adhesive cannot meet all needs, bond strength testing remains one of the key applications used to screen for new products and study the influence of experimental variables. This study was conducted to develop an easy to use method to screen and evaluate tissue adhesives for tissue engineering applications.</p> <p>Method</p> <p>Tissue grips were designed to facilitate the reproducible production of substrate tissue and adhesive strength measurements in universal testing machines. Porcine femoral condyles were used to generate osteochondral test tissue cylinders (substrates) of different shapes. Viability of substrates was tested using PI/FDA staining. Self-bonding properties were determined to examine reusability of substrates (n = 3). Serial measurements (n = 5) in different operation modes (OM) were performed to analyze the bonding strength of tissue adhesives in bone (OM-1) and cartilage tissue either in isolation (OM-2) or under specific requirements in joint repair such as filling cartilage defects with clinical applied fibrin/PLGA-cell-transplants (OM-3) or tissues (OM-4). The efficiency of the method was determined on the basis of adhesive properties of fibrin glue for different assembly times (30 s, 60 s). Seven randomly generated collagen formulations were analyzed to examine the potential of method to identify new tissue adhesives.</p> <p>Results</p> <p>Viability analysis of test tissue cylinders revealed vital cells (>80%) in cartilage components even 48 h post preparation. Reuse (n = 10) of test substrate did not significantly change adhesive characteristics. Adhesive strength of fibrin varied in different test settings (OM-1: 7.1 kPa, OM-2: 2.6 kPa, OM-3: 32.7 kPa, OM-4: 30.1 kPa) and was increasing with assembly time on average (2.4-fold). The screening of the different collagen formulations revealed a substance with significant higher adhesive strength on cartilage (14.8 kPa) and bone tissue (11.8 kPa) compared to fibrin and also considerable adhesive properties when filling defects with cartilage tissue (23.2 kPa).</p> <p>Conclusion</p> <p>The method confirmed adhesive properties of fibrin and demonstrated the dependence of adhesive properties and applied settings. Furthermore the method was suitable to screen for potential adhesives and to identify a promising candidate for cartilage and bone applications. The method can offer simple, replicable and efficient evaluation of adhesive properties in <it>ex vivo</it> specimens and may be a useful supplement to existing methods in clinical relevant settings.</p>
topic Tissue adhesive
Bonding strength
Cartilage
Bone
Transplant
Tissue engineering
Joint repair
Test method
url http://www.biomedcentral.com/1471-2474/13/175
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