Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene

Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene

A 66-year old mild cognitive impairment (MCI) female patient donated her Peripheral blood mononuclear cells (PBMC). PBMC was reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC. The pluripotency of transgene-free iPSCs was confirmed by...

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Main Authors: YI Yan, Yinghui Qiu, Aonan Zhao, Jian Zhao, Ying Wang, Yulei Deng
Format: Article
Language:English
Published: Elsevier 2020-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120300040
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spelling doaj-858c516ed2834d48aa5e5b12083da41a2020-11-25T00:35:53ZengElsevierStem Cell Research1873-50612020-03-0143Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 geneYI Yan0Yinghui Qiu1Aonan Zhao2Jian Zhao3Ying Wang4Yulei Deng5Department of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.Department of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authors.A 66-year old mild cognitive impairment (MCI) female patient donated her Peripheral blood mononuclear cells (PBMC). PBMC was reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers in vitro. Moreover, the iPSC line displayed a normal karyotype. The apolipoprotein E (APOE) ε4 allele, is considered to be the strongest genetic risk factor for Sporadic Alzheimer's disease (AD). Our model might offer a good platform to study the pathological mechanisms and drug testing studies in AD and MCI.http://www.sciencedirect.com/science/article/pii/S1873506120300040
collection DOAJ
language English
format Article
sources DOAJ
author YI Yan
Yinghui Qiu
Aonan Zhao
Jian Zhao
Ying Wang
Yulei Deng
spellingShingle YI Yan
Yinghui Qiu
Aonan Zhao
Jian Zhao
Ying Wang
Yulei Deng
Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene
Stem Cell Research
author_facet YI Yan
Yinghui Qiu
Aonan Zhao
Jian Zhao
Ying Wang
Yulei Deng
author_sort YI Yan
title Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene
title_short Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene
title_full Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene
title_fullStr Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene
title_full_unstemmed Establishment of SIAISi001-A, an induced pluripotent stem cell (iPSC) line from 66-year old mild cognitive impairment (MCI) with two copies of APOE4 gene
title_sort establishment of siaisi001-a, an induced pluripotent stem cell (ipsc) line from 66-year old mild cognitive impairment (mci) with two copies of apoe4 gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-03-01
description A 66-year old mild cognitive impairment (MCI) female patient donated her Peripheral blood mononuclear cells (PBMC). PBMC was reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers in vitro. Moreover, the iPSC line displayed a normal karyotype. The apolipoprotein E (APOE) ε4 allele, is considered to be the strongest genetic risk factor for Sporadic Alzheimer's disease (AD). Our model might offer a good platform to study the pathological mechanisms and drug testing studies in AD and MCI.
url http://www.sciencedirect.com/science/article/pii/S1873506120300040
work_keys_str_mv AT yiyan establishmentofsiaisi001aaninducedpluripotentstemcellipsclinefrom66yearoldmildcognitiveimpairmentmciwithtwocopiesofapoe4gene
AT yinghuiqiu establishmentofsiaisi001aaninducedpluripotentstemcellipsclinefrom66yearoldmildcognitiveimpairmentmciwithtwocopiesofapoe4gene
AT aonanzhao establishmentofsiaisi001aaninducedpluripotentstemcellipsclinefrom66yearoldmildcognitiveimpairmentmciwithtwocopiesofapoe4gene
AT jianzhao establishmentofsiaisi001aaninducedpluripotentstemcellipsclinefrom66yearoldmildcognitiveimpairmentmciwithtwocopiesofapoe4gene
AT yingwang establishmentofsiaisi001aaninducedpluripotentstemcellipsclinefrom66yearoldmildcognitiveimpairmentmciwithtwocopiesofapoe4gene
AT yuleideng establishmentofsiaisi001aaninducedpluripotentstemcellipsclinefrom66yearoldmildcognitiveimpairmentmciwithtwocopiesofapoe4gene
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