ALOE VERA STIMULATES CELL PROLIFERATION, CELL MIGRATION, EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR-A (VEGF-A), AND C-JUN N-TERMINAL KINASE-1 (JNK-1) ON FIBROBLAST CELL CULTURED OF DIABETIC RAT SKIN

• Main Authors: Insanul Firdaus, Nur Arfian, Mae Sri Hartati Wahyuningsih, Denny Agustiningsih
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada 2019-07-01
• Series: Journal of the Medical Sciences
• Subjects: diabetic ulcer; aloe vera; fibroblast cell culture; cell proliferation; cell migration; vegf-a expression; jnk-1 expression
• Online Access: https://jurnal.ugm.ac.id/bik/article/view/39395

ABSTRACT Background: Delayed healing process in diabetic wound caused by the disturbance of cell migration and cell proliferation, also diminished production of VEGF and JNK-1 which are important factors in wound healing process. Aloe vera contains variety of active compounds which can help in wound healing process. The research assess the effect of ethanol extract from A. vera in fibroblast cell cultures of diabetic rat skin by investigating of cell proliferation, cell migration, VEGF-A and JNK-1 expression. Method: Experimental research by using primary skin fibroblast cell culture of diabetic Wistar rat. The samples were given A. vera extract with concentration 500, 250, and 125 µg/mL. The proliferation examination carried out by counting the number of cells, in vitro scratch assay method was used to monitor the cell migration, and RT-PCR was used to VEGF-A and JNK-1 expression examination. Result: Cell proliferation examination in 24 and 48 hour, the AV500 and AV250 group had higher number of cells than negative control group, but there was no significant difference (p>0.05). However the examination in hour 72 had significant difference between AV500 group (29.33±1.28x104 cells/mL) and negative control group (22.91±3.21x104 cells/mL) (p=0.14). The examination of cell migration in 24 hours, the AV500, AV250 and AV125 group had higher percentage of cell migration (78.13±7.18%; 73.88±4.75%; 68.80±17.11%) than negative control group (53.91±2.74%) (p=0.003; p=0.009; and p=0.040). In contrast, examination in 48 and 72 hour had no significant difference (p>0.05). The expression of VEGF-A and JNK-1 in 48 hours, the AV500 group had higher than negative control group (p=0.001 and p=0.003). Conclusion: A.vera has effect to increase cell proliferation, cell migration, VEGF-A and JNK-1 expression in fibroblast cell culture of diabetic rat skin.