In vitro evaluation of Clostridium septicum alpha toxoid

In vitro evaluation of Clostridium septicum alpha toxoid

Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo an...

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Main Authors: F.M. Salvarani, Z.I.P. Lobato, R.A. Assis, C.G.R.D. Lima, R.O.S. Silva, P.S. Pires, F.C.F. Lobato
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2010-08-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
soroneutralização
camundongo
cobaio
cultura de células
vacina
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002&lng=en&tlng=en
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spelling doaj-84511ac81cc84eb49e358fafe5b9007e2020-11-24T23:07:42ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622010-08-0162477878310.1590/S0102-09352010000400002S0102-09352010000400002In vitro evaluation of Clostridium septicum alpha toxoidF.M. Salvarani0Z.I.P. Lobato1R.A. Assis2C.G.R.D. Lima3R.O.S. Silva4P.S. Pires5F.C.F. Lobato6Universidade Federal de Minas GeraisUniversidade Federal de Minas GeraisMinistério da Agricultura, Pecuária e AbastecimentoUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisAiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002&lng=en&tlng=ensoroneutralizaçãocamundongocobaiocultura de célulasvacina
collection DOAJ
language English
format Article
sources DOAJ
author F.M. Salvarani
Z.I.P. Lobato
R.A. Assis
C.G.R.D. Lima
R.O.S. Silva
P.S. Pires
F.C.F. Lobato
spellingShingle F.M. Salvarani
Z.I.P. Lobato
R.A. Assis
C.G.R.D. Lima
R.O.S. Silva
P.S. Pires
F.C.F. Lobato
In vitro evaluation of Clostridium septicum alpha toxoid
Arquivo Brasileiro de Medicina Veterinária e Zootecnia
soroneutralização
camundongo
cobaio
cultura de células
vacina
author_facet F.M. Salvarani
Z.I.P. Lobato
R.A. Assis
C.G.R.D. Lima
R.O.S. Silva
P.S. Pires
F.C.F. Lobato
author_sort F.M. Salvarani
title In vitro evaluation of Clostridium septicum alpha toxoid
title_short In vitro evaluation of Clostridium septicum alpha toxoid
title_full In vitro evaluation of Clostridium septicum alpha toxoid
title_fullStr In vitro evaluation of Clostridium septicum alpha toxoid
title_full_unstemmed In vitro evaluation of Clostridium septicum alpha toxoid
title_sort in vitro evaluation of clostridium septicum alpha toxoid
publisher Universidade Federal de Minas Gerais
series Arquivo Brasileiro de Medicina Veterinária e Zootecnia
issn 1678-4162
publishDate 2010-08-01
description Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.
topic soroneutralização
camundongo
cobaio
cultura de células
vacina
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002&lng=en&tlng=en
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