In vitro evaluation of Clostridium septicum alpha toxoid

In vitro evaluation of Clostridium septicum alpha toxoid

Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo an...

Full description

Bibliographic Details
Main Authors: F.M. Salvarani, Z.I.P. Lobato, R.A. Assis, C.G.R.D. Lima, R.O.S. Silva, P.S. Pires, F.C.F. Lobato
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2010-08-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
soroneutralização
camundongo
cobaio
cultura de células
vacina
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002&lng=en&tlng=en
Description
Summary:Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration.
ISSN:1678-4162

Similar Items