| Summary: | The aim of this study was to assess the major food items ingested by adult specimens of Daphnia laevis within the eutrophic Pampulha reservoir in Belo Horizonte, Minas Gerais, Brazil. The gut content was analyzed after addition of sodium hypochlorite and also through the examination of dissected guts under scanning electron microscopy. The results showed that Chlorophyceae was the main food item ingested, representing c. 80.5% of the total ingested food. Moreover, Eutetramorus fottii, Coelastrum pseudomicroporum and Oocystis lacustris, the dominant phytoplankton species within the reservoir, were the most frequent cells found in the gut contents. Euglenophyta also represented an important food item accounting for 15% of the ingested material, including mainly Trachelomonas volvocina and Euglena oxyuris, although less abundant in the reservoir (< 10% of total phytoplankton). Blue-green algae occurred at much lower percentages in the guts than in the phytoplankton. A small amount of undigested Microcystis aeruginosa colonies were also found in the gut content of D. laevis. Scanning electron microscopy results showed that, besides phytoplankton cells, a great amount of abiogenic material was also ingested. The amount of this inorganic material increased considerably in the tract (from 15% to 75% of the gut content), when a peak of D. laevis was observed in the reservoir. Our assumption is that the ingestion of this inorganic material can be a strategy used by D. laevis to obtain additional food supply.
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