Aspergillus Fumigatus pada Sputum Penderita Batuk Kronik Menggunakan Metode PCR dan Kultur

• Main Authors: Isra Thristy, Yahwardiah Siregar
• Format: Article
• Language: English
• Published: Universitas Padjajaran 2016-06-01
• Series: Majalah Kedokteran Bandung
• Subjects: Aspergillus fumigatus; chronic cough; culture; PCR
• Online Access: http://journal.fk.unpad.ac.id/index.php/mkb/article/view/760
• ISSN: 0126-074X; 2338-6223

The increasing prevalence of fungal growth in humans may occur as a result of the use of various drugs, invasive medical devices, and also the presence of predisposing factors like severe chronic diseases, including malignant disease. Aspergillus fumigatus is a systemic fungal species that can infect human beings. Polymerase chain reaction (PCR) is the most optimum diagnostic method to detect fungus. The purpose of this study was to analyze the presence of Aspergillus fumigatus by using PCR and culture method in sputum of patients with chronic cough. The sample of this study consisted of sputum from 51 patients who visited Haji Adam Malik Hospital in Medan. The examination applied the cross-sectional descriptive method. Sputum was examined using PCR and culture as the gold standard fungal examination. The results from the 51 samples showed that positive PCR results (69%) were found in 35 patients and negative results were found in 16 patients (31%). The culture results showed that positive cultures were seen in 29 samples (57%) and negative cultures were found in 22 samples (49%). it is concluded that from 51 patients with chronic cough sputum, 69% turn out to have Aspergillus fumigatus fungus and this result is reinforced by the culture results. PCR can be used as a quick and precise inspection method for detecting fungus.