Intake of Radionuclides in the Trees of Fukushima Forests 4. Binding of Radioiodine to Xyloglucan

• Main Authors: Miki Nonaka, Chisato Yasukawa, Shoko Aoki, Masateru Itakura, Stefan Willför, Peter Capek, Oded Shoseyov, Masaharu Tsubokura, Kei’ichi Baba, Rumi Kaida, Teruaki Taji, Yoichi Sakata, Takahisa Hayashi
• Format: Article
• Language: English
• Published: MDPI AG 2020-09-01
• Series: Forests
• Subjects: radioiodine gas; forest function; 1,4-linked glucans; poplar seedlings; xyloglucan
• Online Access: https://www.mdpi.com/1999-4907/11/9/957

The 1,4-linked glucans such as xyloglucan and amylose are known to form a complex with iodine/iodide ions and to also be precipitated with CaCl₂ in the presence of iodine. Here, we show that iodine gas could be specifically incorporated into xyloglucan. Furthermore, we show that [¹²⁵I]I₂ gas is, over time, incorporated at high levels into the entire outer surface of poplar seedlings but that spraying seedlings with abscisic acid to close stomata decreases the incorporation of the gas. There was less incorporation of the gas in a transgenic poplar overexpressing xyloglucanase at the early stages when compared with a wild type. This shows that xyloglucan serves as a key absorber of iodine gas into a plant body. After individual leaves of cultured seedlings were exposed to the gas for 30 min, no radioiodine was emitted from those leaves over the following two weeks, indicating that no turnover occurs in radioiodine once it is bound to the polysaccharides in plant tissues. We conclude that forest trees could serve as one of the largest enormous capture systems for the radioiodine fallout following the nuclear power plant accident in Fukushima.