| Summary: | The use of and research on medical cannabis (MC) is becoming more common, yet there are still many challenges regarding plant diseases of this crop. For example, there is a lack of formal and professional knowledge regarding fungi that infect MC plants, and practical and effective methods for managing the casual agents of disease are limited. The purpose of this study was to identify foliar, stem, and soilborne pathogens affecting MC under commercial cultivation in Israel. The predominant major foliage pathogens were identified as <i>Alternaria alternata</i> and <i>Botrytis cinerea</i>, while the common stem and soilborne pathogens were identified as <i>Fusarium oxysporum</i> and <i>F. solani</i>. Other important fungi that were isolated from foliage were those producing various mycotoxins that can directly harm patients, such as <i>Aspergillus</i> spp. and <i>Penicillium</i> spp. The sampling and characterization of potential pathogenic fungi were conducted from infected MC plant parts that exhibited various disease symptoms. Koch postulates were conducted by inoculating healthy MC tissues and intact plants with fungi isolated from infected commercially cultivated symptomatic plants. In this study, we report on the major and most common plant pathogens of MC found in Israel, and determine the seasonal outbreak of each fungus.
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